Anti-JAM-A Antibody (Mouse Monoclonal antibody) General Information
Reacts with: Human
Recombinant Human JAM-A / F11R / CD321 protein (Catalog#10198-H08H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human JAM-A / F11R / CD321 (rh JAM-A / F11R / CD321; Catalog#10198-H08H; NP_058642.1; Met1-Ala242). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #04
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-JAM-A Antibody (Mouse Monoclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-JAM-A Antibody (Mouse Monoclonal antibody) Images
Immunofluorescence staining of Human JAM-A in A431 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human JAM-A monoclonal antibody (1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®594-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to plasma membrane.
Flow cytometric analysis of human JAM-A(CD321) expression on SKBR3 cells. Cells were stained with purified anti-Human JAM-A(CD321), then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Anti-JAM-A Antibody Alternative Names
Anti-CD321 Antibody;Anti-JAM Antibody;Anti-JAM1 Antibody;Anti-JAMA Antibody;Anti-JCAM Antibody;Anti-KAT Antibody;Anti-PAM-1 Antibody
JAM-A Background Information
Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.
Guglielmi KM, et al. (2007) Reovirus binding determinants in junctional adhesion molecule-A. J Biol Chem. 282(24): 17930-40.Yeung D, et al. (2008) Decreased junctional adhesion molecule-A expression during blood-brain barrier breakdown. Acta Neuropathol. 115(6): 635-42.Ong KL, et al. (2009) Elevated plasma level of soluble F11 receptor/junctional adhesion molecule-A (F11R/JAM-A) in hypertension. Am J Hypertens. 22(5): 500-5.