JAM-A / F11R / CD321 Antibody ( PE )

JAM-A / F11R / CD321 Antibody Datasheet

JAM-A / F11R / CD321 Antibody Product Information

• Immunogen: Recombinant Human JAM-A / F11R / CD321 protein (Catalog#10198-H08H)
• Reagents: PE-conjugated mouse monoclonal antibody
• Clone ID: 04
• Ig Type: Mouse IgG1
• Concentration: 10 μl/Test, 0.1 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human JAM-A / F11R / CD321 (rh JAM-A / F11R / CD321; Catalog#10198-H08H; NP_058642.1; Met 1-Ala 242) and conjugated with PE under optimum conditions, the unreacted PE was removed.

JAM-A / F11R / CD321 Antibody Usage Guide

• Specificity: Human JAM-A / F11R / CD321
• Flow Cytometry:
  

  Profile of anti-JAM-A (CD321) reactivity on SKBR3 cells analyzed by flow cytometry.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

JAM-A / F11R / CD321 Antibody Background

As a type Itransmembrane receptor belonging to the immunoglobulin superfamily, the human JAM-A, also known as platelet adhesion molecule 1 (PAM-1) and platelet F11 receptor, is the firstly identified member of the Junctional Adhesion Molecule (JAM) family comprising at least three members. JAM-A is specifically localized in tight junctions of epithelial and endothelial cells and is involved in the regulation of junctional integrity and permeability by serving as a physical barrier in a homophilic manner. JAM-A is also expressed on the surface of hematopoeitic cells, such as platelets and leukocytes, and acts as a ligand for integrin LFA-1 or a platelet receptor, consequently plays key roles in a variety of cellular processes, including platelet aggregation, leukocyte transmigration, and angiogenesis. In addition, JAM-A has also been implicated in the attachment of reovirus that causes diarrhea in human.

References

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7. Barton, E. S. et al., 2001, Cell. 104: 441-451.