Anti-JAM-A Antibody

Cat: 10198-RP04
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Anti-JAM-A Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-JAM-A Antibody
Validated applications
Species reactivity
Reacts with: Human
Human JAM-A
Recombinant Human JAM-A protein (Catalog#10198-H08H)
Produced in rabbits immunized with purified, recombinant Human JAM-A extracellular domain (rh JAM-A; Catalog#10198-H08H; Met 1-Ala 242; NP_058642.1). JAM-A specific IgG was purified by human JAM-A affinity chromatography .
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-JAM-A Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-JAM-A Antibody (Rabbit Polyclonal antibody) Images
Human Junctional Adhesion Molecule A Immunoprecipitation(IP) 7954

JAM-A was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

Lane B:0.5 mg HepG2 Whole Cell Lysate

0.5 µL anti-JAM-A rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-JAM-A rabbit polyclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 39 kDa

Observed band size: 39 kDa

Human Junctional Adhesion Molecule A Western blot (WB) 13140

Anti-JAM-A rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Membrane Lysate

Lysates/proteins at 20 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:33 kDa

Observed band size:38 kDa

(We are unsure as to the identity of these extra bands.)

Anti-JAM-A Antibody Alternative Names
Anti-CD321 Antibody;Anti-JAM Antibody;Anti-JAM1 Antibody;Anti-JAMA Antibody;Anti-JCAM Antibody;Anti-KAT Antibody;Anti-PAM-1 Antibody
JAM-A Background Information

Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.

Full Name
F11 receptor
  • Guglielmi KM, et al. (2007) Reovirus binding determinants in junctional adhesion molecule-A. J Biol Chem. 282(24): 17930-40.
  • Yeung D, et al. (2008) Decreased junctional adhesion molecule-A expression during blood-brain barrier breakdown. Acta Neuropathol. 115(6): 635-42.
  • Ong KL, et al. (2009) Elevated plasma level of soluble F11 receptor/junctional adhesion molecule-A (F11R/JAM-A) in hypertension. Am J Hypertens. 22(5): 500-5.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-JAM-A Antibody (PE) Mouse Monoclonal FCM 10198-MM04-P
    Anti-JAM-A Antibody Rabbit Polyclonal ELISA 10198-T16
    Anti-JAM-A Antibody (APC) Mouse Monoclonal FCM 10198-MM04-A
    Anti-JAM-A Antibody (FITC) Mouse Monoclonal FCM 10198-MM04-F
    Anti-JAM-A Antibody Mouse Monoclonal ELISA,ELISA(Det) 10198-MM02
    Anti-JAM-A Antibody Mouse Monoclonal FCM,ICC/IF,IF 10198-MM04
    Anti-JAM-A Antibody Mouse Monoclonal ELISA(Cap) 10198-MM08
    Anti-JAM-A Antibody Rabbit Polyclonal ELISA 10198-RP01
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