|IP||0.2-1 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
JAM-A was immunoprecipitated using:
Lane A:0.5 mg 293T Whole Cell Lysate
Lane B:0.5 mg HepG2 Whole Cell Lysate0.5 µL anti-JAM-A rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-JAM-A rabbit polyclonal antibody,at 1:1000 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 39 kDa
Observed band size: 39 kDa
Anti-JAM-A rabbit polyclonal antibody at 1:500 dilution
Lane A: HepG2 Membrane LysateLysates/proteins at 20 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:33 kDa
Observed band size:38 kDa
(We are unsure as to the identity of these extra bands.)
Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-JAM-A Antibody (PE)||Mouse||Monoclonal||FCM||10198-MM04-P|
|Anti-JAM-A Antibody (APC)||Mouse||Monoclonal||FCM||10198-MM04-A|
|Anti-JAM-A Antibody (FITC)||Mouse||Monoclonal||FCM||10198-MM04-F|