Anti-JAM-A Antibody

Cat: 10198-RP04
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Anti-JAM-A Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-JAM-A Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human JAM-A
Immunogen
Recombinant Human JAM-A protein (Catalog#10198-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human JAM-A extracellular domain (rh JAM-A; Catalog#10198-H08H; Met 1-Ala 242; NP_058642.1). JAM-A specific IgG was purified by human JAM-A affinity chromatography .
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-JAM-A Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-JAM-A Antibody (Rabbit Polyclonal antibody) Images
Human Junctional Adhesion Molecule A Immunoprecipitation(IP) 7954

JAM-A was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

Lane B:0.5 mg HepG2 Whole Cell Lysate

0.5 µL anti-JAM-A rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-JAM-A rabbit polyclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 39 kDa

Observed band size: 39 kDa

Human Junctional Adhesion Molecule A Western blot (WB) 13140

Anti-JAM-A rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Membrane Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:33 kDa

Observed band size:38 kDa

(We are unsure as to the identity of these extra bands.)

Anti-JAM-A Antibody Alternative Names
Anti-CD321 Antibody;Anti-JAM Antibody;Anti-JAM1 Antibody;Anti-JAMA Antibody;Anti-JCAM Antibody;Anti-KAT Antibody;Anti-PAM-1 Antibody
JAM-A Background Information

Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.

Full Name
F11 receptor
References
  • Guglielmi KM, et al. (2007) Reovirus binding determinants in junctional adhesion molecule-A. J Biol Chem. 282(24): 17930-40.
  • Yeung D, et al. (2008) Decreased junctional adhesion molecule-A expression during blood-brain barrier breakdown. Acta Neuropathol. 115(6): 635-42.
  • Ong KL, et al. (2009) Elevated plasma level of soluble F11 receptor/junctional adhesion molecule-A (F11R/JAM-A) in hypertension. Am J Hypertens. 22(5): 500-5.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-JAM-A Antibody (PE) Mouse Monoclonal FCM 10198-MM04-P
    Anti-JAM-A Antibody Rabbit Polyclonal ELISA 10198-T16
    Anti-JAM-A Antibody (APC) Mouse Monoclonal FCM 10198-MM04-A
    Anti-JAM-A Antibody (FITC) Mouse Monoclonal FCM 10198-MM04-F
    Anti-JAM-A Antibody Mouse Monoclonal ELISA,ELISA(Det) 10198-MM02
    Anti-JAM-A Antibody Mouse Monoclonal FCM,ICC/IF,IF 10198-MM04
    Anti-JAM-A Antibody Mouse Monoclonal ELISA(Cap) 10198-MM08
    Anti-JAM-A Antibody Rabbit Polyclonal ELISA 10198-RP01
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