The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat JAG1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80419-ACG|
|Rat JAG1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80419-ACR|
|Rat JAG1 ORF mammalian expression plasmid, C-Flag tag||RG80419-CF|
|Rat JAG1 ORF mammalian expression plasmid, C-His tag||RG80419-CH|
|Rat JAG1 ORF mammalian expression plasmid, C-Myc tag||RG80419-CM|
|Rat JAG1 ORF mammalian expression plasmid, C-HA tag||RG80419-CY|
|Rat JAG1 ORF mammalian expression plasmid, N-Flag tag||RG80419-NF|
|Rat JAG1 ORF mammalian expression plasmid, N-His tag||RG80419-NH|
|Rat JAG1 ORF mammalian expression plasmid, N-Myc tag||RG80419-NM|
|Rat JAG1 ORF mammalian expression plasmid, N-HA tag||RG80419-NY|
|Rat JAG1 natural ORF mammalian expression plasmid||RG80419-UT|
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Protein Jagged 1, also known as JAG1, JAGL1 and CD339, is a single-pass type I membrane protein which contains 1 DSL domain and 15 EGF-like domains. JAG1/Jagged 1 is widely expressed in adult and fetal tissues. The expression of JAG1/Jagged 1 is up-regulated in cervical squamous cell carcinoma. JAG1/Jagged 1 is also expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells. JAG1/Jagged 1 is a ligand for multiple Notch receptors. It is involved in the mediation of Notch signaling. JAG1/Jagged 1 may be involved in cell-fate decisions during hematopoiesis.
JAG1/Jagged 1 seems to be involved in early and late stages of mammalian cardiovascular development. It inhibits myoblast differentiation and enhances fibroblast growth factor-induced angiogenesis. Defects in JAG1/Jagged 1 are the cause of Alagille syndrome type 1 (ALGS1). Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. Defects in JAG1/Jagged 1 are also a cause of tetralogy of Fallot (TOF). TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation.