Rat JAG1 Gene cDNA Clone / ORF Clone

JAG1 cDNA Clone Datasheet

JAG1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_019147.1
• cDNA Size: 3660
• Gene Synonym: Jag1
• Source: Rat

JAG1 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus jagged 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 3557 T/C，3562-3564 CAG/AGC, 3565 C/G, resulting in the amino acid Val substitution by Ala, Gln substitution by Ser, Arg substitution by Gly and 636 C/T,1761 T/G，1944 T/C not causing the amino acid variation.

JAG1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

JAG1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

JAG1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGGTCCC CACGGACGCG CGGCCGGCCG GGGCGCCCCT TGAGCCTTCT GCTCGCCCTG
61       CTCTGTGCCC TGCGAGCCAA GGTGTGCGGG GCCTCGGGTC AATTTGAGCT GGAGATCCTG
121      TCCATGCAGA ACGTGAACGG AGAGTTACAG AATGGGAACT GTTGCGGCGG AGCCCGGAAC
181      CCTGGCGACC GCAAGTGCAC CCGCGACGAG TGTGACACGT ACTTCAAAGT GTGCCTCAAG
241      GAGTATCAGT CCCGCGTCAC GGCCGGGGGA CCCTGCAGCT TCGGCTCAGG GTCTACGCCT
301      GTCATCGGGG GCAATACCTT CAATCTCAAG GCCAGCCGCG GCAACGACCG TAATCGCATC
361      GTACTGCCTT TCAGTTTCGC CTGGCCGAGG TCCTACACTT TGCTGGTGGA GGCATGGGAT
421      TCCAGTAATG ACACTATTCA ACCTGATAGC ATAATTGAAA AGGCTTCTCA CTCAGGCATG
481      ATAAACCCCA GCCGGCAGTG GCAGACACTG AAACAGAACA CAGGGATTGC CCACTTTGAG
541      TATCAGATCC GAGTGACCTG TGATGACCAC TACTATGGCT TTGGCTGCAA CAAGTTCTGT
601      CGGCCCAGAG ATGACTTCTT TGGACATTAT GCCTGTGACC AGAATGGCAA CAAAACCTGC
661      ATGGAAGGCT GGATGGGTCC TGAGTGTAAC AAAGCAATAT GCCGACAGGG CTGCAGTCCC
721      AAGCATGGCT CCTGCAAACT CCCAGGTGAC TGCAGGTGCC AGTATGGTTG GCAGGGCCTG
781      TACTGTGATA AGTGCATCCC GCACCCAGGA TGTGTCCACG GCACCTGCAA TGAACCCTGG
841      CAGTGCCTCT GTGAAACCAA CTGGGGTGGA CAGCTCTGTG ACAAAGATCT GAATTACTGT
901      GGGACTCATC AGCCCTGTCT CAACCGGGGA ACATGTAGCA ACACTGGCCC TGACAAATAC
961      CAGTGTTCCT GCCCCGAGGG CTACTCAGGA CCGAACTGTG AAATTGCTGA GCATGCCTGC
1021     CTCTCTGATC CCTGCCACAA CAGAGGCAGC TGCAAGGAGA CCTCCTCGGG CTTTGAGTGT
1081     GAGTGTTCTC CAGGCTGGAC TGGACCCACG TGTTCCACAA ACATCGATGA CTGTTCTCCA
1141     AATAACTGTT CCCATGGAGG CACCTGCCAG GATCTAGTGA ATGGATTCAA GTGCGTGTGC
1201     CCACCCCAGT GGACTGGCAA GACTTGTCAG TTAGATGCAA ATGAGTGCGA GGCCAAGCCT
1261     TGTGTAAATG CCAGATCCTG TAAGAATCTG ATTGCCAGCT ACTACTGTGA TTGTCTTCCT
1321     GGCTGGATGG GTCAGAATTG TGACATAAAT ATCAATGACT GCCTTGGCCA GTGTCAGAAT
1381     GATGCCTCCT GTCGGGATTT GGTTAATGGT TATCGCTGTA TCTGTCCACC TGGCTATGCG
1441     GGCGATCACT GTGAAAGAGA CATCGATGAA TGTGCTAGCA ACCCCTGCTT GAATGGGGGT
1501     CACTGTCAGA ATGAAATTAA CAGATTCCAG TGTCTGTGTC CCACTGGTTT CTCTGGAAAC
1561     CTCTGTCAGC TGGACATCGA TTACTGCGAG CCCAACCCTT GCCAGAATGG CGCCCAATGC
1621     TACAATCGTG CCAGTGACTA TTTCTGCAAG TGCCCTGAGG ACTACGAGGG CAAGAACTGC
1681     TCACACCTGA AAGACCACTG CCGCACCACT CCCTGTGAAG TGATTGACAG CTGCACCGTG
1741     GCCATGGCCT CCAACGATAC GCCTGAAGGG GTGCGGTATA TCTCTTCCAA CGTCTGTGGT
1801     CCCCATGGGA AGTGCAAGAG CGAGTCAGGA GGCAAATTCA CCTGTGACTG TAACAAAGGC
1861     TTCACCGGCA CCTACTGCCA TGAAAATATC AACGACTGTG AGGGCAACCC CTGTACGAAC
1921     GGTGGCACGT GCATCGACGG CGTCAACTCC TACAAGTGTA TCTGTAGTGA CGGCTGGGAG
1981     GGCGCGCACT GTGAGAACAA CATAAATGAC TGTAGCCAGA ACCCTTGCCA CTATGGGGGT
2041     ACATGTCGAG ACCTGGTCAA TGACTTTTAC TGTGACTGCA AAAATGGGTG GAAAGGGAAA
2101     ACCTGCCACT CGCGTGACAG CCAGTGTGAC GAAGCCACGT GTAATAACGG TGGTACCTGC
2161     TATGATGAAG TGGATACGTT TAAGTGCATG TGTCCTGGTG GCTGGGAAGG AACAACCTGT
2221     AATATAGCTA GAAACAGTAG CTGCCTGCCG AACCCCTGTC ATAATGGAGG CACCTGCGTG
2281     GTCAATGGAG ACTCCTTCAC CTGTGTCTGC AAAGAAGGCT GGGAGGGGCC GATTTGTACT
2341     CAAAATACCA ACGACTGCAG TCCTCACCCT TGTTACAACA GCGGAACCTG TGTGGATGGA
2401     GATAACTGGT ACCGGTGCGA ATGTGCCCCT GGTTTTGCTG GGCCAGACTG CAGGATAAAC
2461     ATCAATGAGT GTCAGTCTTC CCCTTGTGCC TTTGGGGCCA CCTGTGTGGA TGAGATCAAT
2521     GGCTACCAGT GTATCTGCCC TCCAGGACAT AGTGGTGCCA AGTGCCATGA AGTTTCAGGG
2581     AGATCTTGCA TCACCATGGG GAGAGTGATA CTTGATGGGG CCAAGTGGGA TGACGACTGT
2641     AACACCTGCC AGTGCCTGAA TGGACGGGTG GCCTGCTCCA AGGTCTGGTG TGGCCCGAGA
2701     CCTTGCCGGC TCCACAAAGG GCACGGCGAG TGTCCCAATG GGCAGAGCTG CATCCCAGTC
2761     CTGGATGACC AGTGTTTCGT GCGCCCCTGC ACTGGTGCTG GCGAGTGTCG GTCCTCCAGC
2821     CTCCAGCCAG TGAAGACCAA GTGTACATCG GACTCTTATT ACCAGGACAA TTGTGCGAAC
2881     ATCACTTTCA CCTTTAACAA GGAGATGATG TCTCCAGGTC TCACCACTGA GCACATTTGC
2941     AGCGAATTGA GGAATCTGAA TATTCTGAAG AATGTTTCTG CTGAGTATTC AATCTACATA
3001     GCCTGTGAGC CTTCCTTATC AGCAAACAAT GAAATACACG TGGCCATTTC TGCCGAAGAC
3061     ATCCGGGATG ATGGAAACCC TGTCAAGGAA ATTACTGATA AAATAATAGA TCTCGTTAGT
3121     AAACGGGATG GGAACAGCTC ACTTATCGCT GCTGTTGCAG AAGTCAGAGT TCAGAGGCGC
3181     CCTCTGAAAA ACAGAACAGA TTTCCTGGTT CCTCTGCTGA GCTCTGTCTT AACAGTGGCT
3241     TGGGTCTGTT GCTTGGTGAC AGCCTTCTAC TGGTGTGTGC GGAAGCGGCG GCGGAAGCCC
3301     AGCAGCCACA CCCACTCTGC CCCTGAGGAC AACACCACCA ACAATGTGCG CGAACAGCTG
3361     AACCAAATCA AAAACCCCAT CGAGAAACAC GGAGCCAACA CAGTCCCCAT CAAGGATTAT
3421     GAGAACAAAA ACTCCAAAAT GTCAAAAATC AGGACACACA ACTCGGAAGT GGAAGAGGAT
3481     GATATGGATA AGCACCAGCA GAAAGTCCGG TTTGCCAAAC AGCCCGTGTA CACGCTGGTA
3541     GACAGAGAGG AGAAGGCCCC CAGCGGCACG CCGACAAAAC ACCCGAACTG GACAAACAAA
3601     CAGGACAACA GAGACTTGGA AAGTGCCCAG AGCTTGAACC GGATGGAGTA CATCGTATAG

 

Quality Control:cDNA sequencing results is available upon request.