Human JAG1 ORF Expression Clone, Myc-tagged

JAG1 cDNA Clone Datasheet

JAG1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_000214.2
• cDNA Size: 3687
• Gene Synonym: AGS; AHD; AWS; HJ1; CD339; JAGL1; MGC104644; JAG1
• Source: Human

JAG1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens collagen triple helix repeat containing 1 DNA.
• Vector: pCMV/hygro-Myc
• Myc Tag Sequence: CAGAAACTCATCTCAGAAGAGGATCTG
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for four point mutations: 402 G/A, 2526 C/T and 3417 T/C not causing the amino acid variation

JAG1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

JAG1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

JAG1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGTTCCC CACGGACGCG CGGCCGGTCC GGGCGCCCCC TAAGCCTCCT GCTCGCCCTG
61       CTCTGTGCCC TGCGAGCCAA GGTGTGTGGG GCCTCGGGTC AGTTCGAGTT GGAGATCCTG
121      TCCATGCAGA ACGTGAACGG GGAGCTGCAG AACGGGAACT GCTGCGGCGG CGCCCGGAAC
181      CCGGGAGACC GCAAGTGCAC CCGCGACGAG TGTGACACAT ACTTCAAAGT GTGCCTCAAG
241      GAGTATCAGT CCCGCGTCAC GGCCGGGGGG CCCTGCAGCT TCGGCTCAGG GTCCACGCCT
301      GTCATCGGGG GCAACACCTT CAACCTCAAG GCCAGCCGCG GCAACGACCG CAACCGCATC
361      GTGCTGCCTT TCAGTTTCGC CTGGCCGAGG TCCTATACGT TACTTGTGGA GGCGTGGGAT
421      TCCAGTAATG ACACCGTTCA ACCTGACAGT ATTATTGAAA AGGCTTCTCA CTCGGGCATG
481      ATCAACCCCA GCCGGCAGTG GCAGACGCTG AAGCAGAACA CGGGCGTTGC CCACTTTGAG
541      TATCAGATCC GCGTGACCTG TGATGACTAC TACTATGGCT TTGGCTGCAA TAAGTTCTGC
601      CGCCCCAGAG ATGACTTCTT TGGACACTAT GCCTGTGACC AGAATGGCAA CAAAACTTGC
661      ATGGAAGGCT GGATGGGCCC CGAATGTAAC AGAGCTATTT GCCGACAAGG CTGCAGTCCT
721      AAGCATGGGT CTTGCAAACT CCCAGGTGAC TGCAGGTGCC AGTACGGCTG GCAAGGCCTG
781      TACTGTGATA AGTGCATCCC ACACCCGGGA TGCGTCCACG GCATCTGTAA TGAGCCCTGG
841      CAGTGCCTCT GTGAGACCAA CTGGGGCGGC CAGCTCTGTG ACAAAGATCT CAATTACTGT
901      GGGACTCATC AGCCGTGTCT CAACGGGGGA ACTTGTAGCA ACACAGGCCC TGACAAATAT
961      CAGTGTTCCT GCCCTGAGGG GTATTCAGGA CCCAACTGTG AAATTGCTGA GCACGCCTGC
1021     CTCTCTGATC CCTGTCACAA CAGAGGCAGC TGTAAGGAGA CCTCCCTGGG CTTTGAGTGT
1081     GAGTGTTCCC CAGGCTGGAC CGGCCCCACA TGCTCTACAA ACATTGATGA CTGTTCTCCT
1141     AATAACTGTT CCCACGGGGG CACCTGCCAG GACCTGGTTA ACGGATTTAA GTGTGTGTGC
1201     CCCCCACAGT GGACTGGGAA AACGTGCCAG TTAGATGCAA ATGAATGTGA GGCCAAACCT
1261     TGTGTAAACG CCAAATCCTG TAAGAATCTC ATTGCCAGCT ACTACTGCGA CTGTCTTCCC
1321     GGCTGGATGG GTCAGAATTG TGACATAAAT ATTAATGACT GCCTTGGCCA GTGTCAGAAT
1381     GACGCCTCCT GTCGGGATTT GGTTAATGGT TATCGCTGTA TCTGTCCACC TGGCTATGCA
1441     GGCGATCACT GTGAGAGAGA CATCGATGAA TGTGCCAGCA ACCCCTGTTT GAATGGGGGT
1501     CACTGTCAGA ATGAAATCAA CAGATTCCAG TGTCTGTGTC CCACTGGTTT CTCTGGAAAC
1561     CTCTGTCAGC TGGACATCGA TTATTGTGAG CCTAATCCCT GCCAGAACGG TGCCCAGTGC
1621     TACAACCGTG CCAGTGACTA TTTCTGCAAG TGCCCCGAGG ACTATGAGGG CAAGAACTGC
1681     TCACACCTGA AAGACCACTG CCGCACGACC CCCTGTGAAG TGATTGACAG CTGCACAGTG
1741     GCCATGGCTT CCAACGACAC ACCTGAAGGG GTGCGGTATA TTTCCTCCAA CGTCTGTGGT
1801     CCTCACGGGA AGTGCAAGAG TCAGTCGGGA GGCAAATTCA CCTGTGACTG TAACAAAGGC
1861     TTCACGGGAA CATACTGCCA TGAAAATATT AATGACTGTG AGAGCAACCC TTGTAGAAAC
1921     GGTGGCACTT GCATCGATGG TGTCAACTCC TACAAGTGCA TCTGTAGTGA CGGCTGGGAG
1981     GGGGCCTACT GTGAAACCAA TATTAATGAC TGCAGCCAGA ACCCCTGCCA CAATGGGGGC
2041     ACGTGTCGCG ACCTGGTCAA TGACTTCTAC TGTGACTGTA AAAATGGGTG GAAAGGAAAG
2101     ACCTGCCACT CACGTGACAG TCAGTGTGAT GAGGCCACGT GCAACAACGG TGGCACCTGC
2161     TATGATGAGG GGGATGCTTT TAAGTGCATG TGTCCTGGCG GCTGGGAAGG AACAACCTGT
2221     AACATAGCCC GAAACAGTAG CTGCCTGCCC AACCCCTGCC ATAATGGGGG CACATGTGTG
2281     GTCAACGGCG AGTCCTTTAC GTGCGTCTGC AAGGAAGGCT GGGAGGGGCC CATCTGTGCT
2341     CAGAATACCA ATGACTGCAG CCCTCATCCC TGTTACAACA GCGGCACCTG TGTGGATGGA
2401     GACAACTGGT ACCGGTGCGA ATGTGCCCCG GGTTTTGCTG GGCCCGACTG CAGAATAAAC
2461     ATCAATGAAT GCCAGTCTTC ACCTTGTGCC TTTGGAGCGA CCTGTGTGGA TGAGATCAAT
2521     GGCTATCGGT GTGTCTGCCC TCCAGGGCAC AGTGGTGCCA AGTGCCAGGA AGTTTCAGGG
2581     AGACCTTGCA TCACCATGGG GAGTGTGATA CCAGATGGGG CCAAATGGGA TGATGACTGT
2641     AATACCTGCC AGTGCCTGAA TGGACGGATC GCCTGCTCAA AGGTCTGGTG TGGCCCTCGA
2701     CCTTGCCTGC TCCACAAAGG GCACAGCGAG TGCCCCAGCG GGCAGAGCTG CATCCCCATC
2761     CTGGACGACC AGTGCTTCGT CCACCCCTGC ACTGGTGTGG GCGAGTGTCG GTCTTCCAGT
2821     CTCCAGCCGG TGAAGACAAA GTGCACCTCT GACTCCTATT ACCAGGATAA CTGTGCGAAC
2881     ATCACATTTA CCTTTAACAA GGAGATGATG TCACCAGGTC TTACTACGGA GCACATTTGC
2941     AGTGAATTGA GGAATTTGAA TATTTTGAAG AATGTTTCCG CTGAATATTC AATCTACATC
3001     GCTTGCGAGC CTTCCCCTTC AGCGAACAAT GAAATACATG TGGCCATTTC TGCTGAAGAT
3061     ATACGGGATG ATGGGAACCC GATCAAGGAA ATCACTGACA AAATAATCGA TCTTGTTAGT
3121     AAACGTGATG GAAACAGCTC GCTGATTGCT GCCGTTGCAG AAGTAAGAGT TCAGAGGCGG
3181     CCTCTGAAGA ACAGAACAGA TTTCCTTGTT CCCTTGCTGA GCTCTGTCTT AACTGTGGCT
3241     TGGATCTGTT GCTTGGTGAC GGCCTTCTAC TGGTGCCTGC GGAAGCGGCG GAAGCCGGGC
3301     AGCCACACAC ACTCAGCCTC TGAGGACAAC ACCACCAACA ACGTGCGGGA GCAGCTGAAC
3361     CAGATCAAAA ACCCCATTGA GAAACATGGG GCCAACACGG TCCCCATCAA GGATTACGAG
3421     AACAAGAACT CCAAAATGTC TAAAATAAGG ACACACAATT CTGAAGTAGA AGAGGACGAC
3481     ATGGACAAAC ACCAGCAGAA AGCCCGGTTT GCCAAGCAGC CGGCGTACAC GCTGGTAGAC
3541     AGAGAAGAGA AGCCCCCCAA CGGCACGCCG ACAAAACACC CAAACTGGAC AAACAAACAG
3601     GACAACAGAG ACTTGGAAAG TGCCCAGAGC TTAAACCGAA TGGAGTACAT CGTAGCT1CAG
3661     AAACTCATCT CAGAAGAGGA TCTG2TAA

 

1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. Myc Tag

 

 

Quality Control:cDNA sequencing results is available upon request.