Rat Interleukin 25 / IL25 / IL17E Protein (Cytokine)

Interleukin 25 Protein Datasheet

Interleukin 25 / IL25 / IL17E Protein Product Information

• Synonym: IL25
• Protein Construction: A DNA sequence encoding the rat IL25 (D3ZLB1)(Val17-Ala169) was expressed, fused with Fc region of human IgG₁ at the N-terminus.
• Source: Rat
• Expression Host: Human Cells

Interleukin 25 / IL25 / IL17E Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu
• Molecular Mass: The recombinant rat IL25 comprises 413 amino acids and predicts a molecular mass of 46.1 kDa. The apparent molecular mass of the recombinant protein is approximately 49 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: Interleukin 25 / IL25 / IL17E protein

Interleukin 25 / IL25 / IL17E Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Interleukin 25 / IL25 / IL17E Protein Description

Interleukin 25 (IL-25), also known as IL17E, is a cytokine that belongs to the IL17 cytokine family and is secreted by type 2 helper T cells (Th2) and mast cells.  IL-25 induces the production of other cytokines, including IL-4, IL-5 and IL-13 in multiple tissues, then induces Th2 immune responses such as blood eosinophilia and increased serum immunoglobulin E levels.This cytokine is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract. Further, the IL-25 gene has been identified in a chromosomal region associated with autoimmune diseases of the gut such as inflammatory bowel disease (IBD). In addition, studies shown that enforced expression of IL-25 in the lung significantly enhances antigen-induced Th2 cytokine production and eosinophil recruitment into the airways, thus suggesting an important role of IL-25in enhancing allergic airway inflammation by a CD4+ T-cell-dependent mechanism.

References

1. Kei I. et al., 2003, Blood. 101: 3594-6.
2. Buning. et al., 2003, European Journal of Immunogenetics. 30: 329-33.
3. Owyang. et al., 2006, Journal of Experimental Medicine. 203: 843-9.
4. Tamachi T. et al., 2006, Int Arch Allergy Immunol. 1: 59-62.
5. Angkasekwinai P. et al., 2007, J Exp Med. 204: 1509-17.