Influenza B (B/Malaysia/2506/2004) Hemagglutinin Gene cDNA Clone / ORF Clone (codon optimized)

Influenza B (B/Malaysia/2506/2004) cDNA Clone Datasheet

Influenza B (B/Malaysia/2506/2004) cDNA Clone Product Information

• cDNA Size: 1758
• Gene Synonym: HA1, Hemagglutinin
• Source: Influenza Virus

Influenza B (B/Malaysia/2506/2004) cDNA Clone Description

• cDNA Description: ORF Clone of Influenza B (B/Malaysia/2506/2004) HA DNA.

  This cDNA clone has gone through customized codon optimization in order to obtain high level of protein expression in particular cell lines. Therefore, although the translated amino acid sequence is identical to the amino sequence on Gene Bank, the DNA sequence is different from that on Gene Bank.

• Vector: Vector 3. Restriction site: Hind III + XbaI

Influenza B (B/Malaysia/2506/2004) cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza B (B/Malaysia/2506/2004) cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza B (B/Malaysia/2506/2004) cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGGCTA TCATTGTGCT GCTGATGGTG GTGACCAGCA ATGCTGACAG GATTTGTACT
61       GGCATCACCT CCAGCAACAG CCCTCATGTG GTGAAGACAG CCACCCAGGG AGAGGTGAAT
121      GTGACAGGAG TGATTCCACT GACCACCACA CCAACCAAGA GCCACTTTGC CAACCTGAAA
181      GGCACAGAGA CCAGGGGCAA ACTGTGTCCA AAGTGTCTGA ACTGTACTGA CCTGGATGTG
241      GCTCTGGGCA GACCAAAGTG TACTGGCAAC ATCCCATCTG CCAGGGTGAG CATCCTGCAT
301      GAGGTGAGAC CTGTGACCTC TGGCTGTTTT CCAATAATGC ATGACAGGAC CAAGATTAGA
361      CAACTTCCAA ACCTGCTGAG GGGCTATGAA CACATCAGAC TGAGCACCCA CAATGTGATA
421      AATGCTGAGA ATGCCCCTGG AGGACCATAC AAGATTGGCA CCTCTGGCTC CTGTCCAAAT
481      GTGACCAATG GCAATGGCTT CTTTGCCACA ATGGCTTGGG CTGTGCCAAA GAATGACAAC
541      AACAAGACAG CCACCAACTC CCTGACCATT GAGGTGCCAT ACATCTGTAC TGAGGGAGAG
601      GACCAGATTA CAGTGTGGGG CTTCCACTCT GACAATGAGA TTCAGATGGC TAAACTCTAT
661      GGAGACAGCA AGCCACAGAA GTTCACCTCC TCTGCCAATG GAGTGACCAC CCACTATGTG
721      AGCCAGATTG GAGGCTTTCC AAACCAGACA GAGGATGGAG GACTGCCACA GTCTGGCAGG
781      ATTGTGGTGG ACTATATGGT CCAGAAGTCT GGCAAGACAG GCACCATCAC CTACCAGAGG
841      GGCATCCTGC TGCCACAGAA GGTGTGGTGT GCCTCTGGCA GGAGCAAGGT GATTAAGGGC
901      TCCCTGCCAC TGATTGGAGA GGCTGACTGT CTGCATGAGA AATATGGAGG ACTGAACAAG
961      AGCAAGCCAT ACTACACAGG AGAACATGCC AAGGCTATTG GCAACTGTCC AATCTGGGTG
1021     AAGACACCAC TGAAACTGGC TAATGGCACC AAATACAGAC CTCCTGCCAA ACTGCTGAAA
1081     GAGAGGGGCT TCTTTGGAGC CATTGCTGGC TTCTTGGAGG GAGGCTGGGA GGGGATGATT
1141     GCTGGCTGGC ATGGCTACAC CTCTCATGGA GCTCATGGAG TGGCTGTGGC TGCTGACCTG
1201     AAAAGCACCC AGGAGGCTAT CAACAAGATT ACCAAGAACC TGAACTCCCT GTCTGAATTG
1261     GAGGTGAAGA ACCTCCAAAG ACTGTCTGGA GCTATGGATG AACTGCACAA TGAGATTTTG
1321     GAACTGGATG AGAAGGTGGA TGACCTGAGG GCTGACACCA TCTCCAGCCA GATTGAACTG
1381     GCTGTGCTGC TGAGCAATGA GGGCATCATC AACTCTGAGG ATGAACACCT GCTGGCTTTG
1441     GAGAGGAAAC TGAAAAAGAT GCTGGGACCA TCTGCTGTGG AGATTGGCAA TGGCTGTTTT
1501     GAGACCAAAC ACAAGTGTAA CCAGACTTGT CTGGACAGGA TTGCTGCTGG CACCTTTGAT
1561     GCTGGAGAGT TCTCCCTGCC AACCTTTGAC TCCCTGAACA TCACAGCAGC CTCCCTGAAT
1621     GATGATGGAC TGGACAACCA CACCATCCTG CTCTACTACA GCACAGCAGC CTCCTCCCTG
1681     GCTGTGACCC TGATGATTGC CATCTTTGTG GTCTATATGG TGAGCAGGGA CAATGTGTCC
1741     TGTAGCATCT GTCTGTAA

 

Quality Control:cDNA sequencing results is available upon request.