+86-400-890-9989
Product Catalog
Influenza B Hemagglutinin (HA) ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK11053 |
|
YES | 
|
    |
|
Influenza B HA / Hemagglutinin ELISA Pair Set
 |
Order or Inquire for Influenza B HA / Hemagglutinin ELISA Pair Set |
|
|
High affinity Influenza B HA / Hemagglutinin ELISA Pair Set |
|
|
Detection limit - 62.5 pg/ml |
|
|
Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
|
|
|
|
|
No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
|
Substrate solution was not added |
Add substrate solution and continue |
|
|
Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
|
|
Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
|
Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
|
|
Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
|
|
Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
|
|
Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
|
Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
|
|
High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
|
Increase cycles of washes and soaking time between washes |
||
|
TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
|
|
Materials were contaminated |
Use clean plates, tubes and pipettes tips |
|
|
Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
|
The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Influenza B HA / Hemagglutinin ELISA Pair Set Related Products & Topics
Background
Influenza (flu) is a respiratory infection in mammals and birds. This virus is divided into three main types (A, B and C). Influenza A is found in a wide variety of bird and mammal species. Influenza B is largely confined to humans and is an important cause of morbidity. Influenza C infects humans, dogs and pigs, sometimes causing both severe illness and local epidemics. Influenza A is further divided into subtypes based on differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA). The notation HhNn is used to refer to the subtype comprising the hth discovered HA protein and the nth discovered NA protein. The HA is a trimer with a receptor binding pocket on the globular head of each monomer. Subtypes are further divided into strains. Each genetically distinct virus isolate is usually considered to be a separate strain. Influenza B viruses are only known to infect humans and seals, giving them influenza. This limited host range is apparently responsible for the lack of Influenza virus B caused influenza pandemics in contrast with those caused by the morphologically similar Influenza virus A as both mutate by both genetic drift and reassortment. Influenza B viruses evolve slower than A viruses and faster than C viruses. Influenza B virus mutates at a rate 2 to 3 times lower than type A. However, influenza B mutates enough that lasting immunity is not possible.
