Influenza A H9N2 (A/Hong Kong/1073/99) Hemagglutinin partial cDNA Clone / ORF Clone (codon optimized)

Influenza A H9N2 (A/Hong Kong/1073/99) Hemagglutinin cDNA Clone Datasheet

Influenza A H9N2 (A/Hong Kong/1073/99) Hemagglutinin cDNA Clone Product Information

• cDNA Size: 1605
• Gene Synonym: HA1, Hemagglutinin
• Source: Influenza Virus

Influenza A H9N2 (A/Hong Kong/1073/99) Hemagglutinin cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H9N2 (A/Hong Kong/1073/99) HA DNA.

  This cDNA clone has gone through customized codon optimization in order to obtain high level of protein expression in particular cell lines. Therefore, although the translated amino acid sequence is identical to the amino sequence on Gene Bank, the DNA sequence is different from that on Gene Bank.

• Vector: Vector 3. Restriction site: KpnI + XbaI
• His Tag Sequence: CATCACCACCATCACCACCATCATCACCAT

Influenza A H9N2 (A/Hong Kong/1073/99) Hemagglutinin cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H9N2 (A/Hong Kong/1073/99) Hemagglutinin cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H9N2 (A/Hong Kong/1073/99) Hemagglutinin cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGACCA TCTCCCTGAT TACCATCCTG CTGGTGGTGA CAGCCAGCAA TGCTGACAAG
61       ATTTGTATTG GACACCAGAG CACCAACAGC ACAGAGACAG TGGACACCCT GACAGAGACC
121      AATGTGCCTG TGACCCATGC CAAGGAACTG CTGCACACAG AACACAATGG GATGCTGTGT
181      GCCACCTCCC TGGGACACCC ACTGATTCTG GACACTTGTA CCATTGAGGG ACTGGTCTAT
241      GGCAACCCAT CCTGTGACCT GCTGCTGGGA GGCAGGGAGT GGTCCTACAT TGTGGAGAGG
301      TCCTCTGCTG TGAATGGCAC TTGTTACCCT GGCAATGTGG AGAACTTGGA GGAACTGAGG
361      ACCCTGTTCT CCTCTGCCTC CTCCTACCAG AGGATTCAGA TTTTTCCTGA CACCACCTGG
421      AATGTGACCT ACACAGGCAC CAGCAGGGCT TGTTCTGGCT CCTTCTACAG GAGTATGAGG
481      TGGCTGACCC AGAAGTCTGG CTTCTACCCT GTCCAGGATG CCCAATACAC CAACAACAGG
541      GGCAAGAGCA TCCTGTTTGT GTGGGGCATC CACCACCCTC CAACCTACAC AGAACAGACC
601      AACCTCTACA TCAGGAATGA CACCACCACC TCTGTGACCA CAGAGGACCT GAACAGGACC
661      TTCAAGCCTG TGATTGGACC AAGACCACTG GTGAATGGAC TCCAAGGCAG GATTGACTAC
721      TACTGGTCTG TGCTGAAACC TGGACAAACC CTGAGGGTGA GGAGCAATGG CAACCTGATT
781      GCCCCATGGT ATGGTCATGT GCTGTCTGGA GGCTCTCATG GCAGGATTCT GAAAACAGAC
841      CTGAAAGGAG GCAACTGTGT GGTCCAGTGT CAGACAGAGA AGGGAGGACT GAACAGCACC
901      CTGCCATTCC ACAACATCAG CAAATATGCC TTTGGCACTT GTCCAAAATA TGTGAGGGTG
961      AACTCCCTGA AACTGGCTGT GGGACTGAGG AATGTGCCTG CCAGGTCCAG CAGGGGACTG
1021     TTTGGAGCCA TTGCTGGCTT CATTGAGGGA GGCTGGCCTG GACTGGTGGC TGGCTGGTAT
1081     GGCTTCCAAC ACAGCAATGA CCAGGGAGTG GGGATGGCTG CTGACAGGGA CAGCACCCAG
1141     AAGGCTATTG ACAAGATTAC CAGCAAGGTG AACAACATTG TGGACAAGAT GAACAAGCAA
1201     TATGAGATTA TTGACCATGA GTTCTCTGAG GTGGAGACCA GACTGAATAT GATAAACAAC
1261     AAGATTGATG ACCAGATTCA GGATGTGTGG GCTTACAATG CTGAACTGCT GGTGCTGTTG
1321     GAGAACCAGA AGACCCTGGA TGAACATGAT GCCAATGTGA ACAACCTCTA CAACAAGGTG
1381     AAGAGGGCTC TGGGCAGCAA TGCTATGGAG GATGGCAAGG GCTGTTTTGA ACTCTACCAC
1441     AAGTGTGATG ACCAGTGTAT GGAGACCATC AGGAATGGCA CCTACAACAG GAGGAAATAC
1501     AGGGAGGAGA GCAGATTGGA GAGACAGAAG ATTGAGGGAG TGAAATTGGA GTCTGAGGGC
1561     ACCTACAAGG CT1CATCACCA CCATCACCAC CATCATCACC AT2TAA

 

1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. 10 × His Tag

 

Quality Control:cDNA sequencing results is available upon request.