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Influenza A H9N2 (A/brambling/Beijing/16/2012) polymerase Pb1 (Codon Optimized) ORF mammalian expression plasmid, N-His tag

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H9N2 PB1 cDNA Clone Product Information
NCBI RefSeq:
RefSeq ORF Size:2274bp
cDNA Description:Full length Clone DNA of Influenza A H9N2 (A/brambling/Beijing/16/2012) polymerase Pb1 with N terminal His tag.
Gene Synonym:PB1
Species:H9N2
Vector:pCMV3-N-His
Plasmid:pCMV3-His-H9N2-BB-12-PB1
Restriction Site:KpnI + XbaI (6kb + 2.32kb)
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with AGE15506.1.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
H9N2 PB1 Gene Plasmid Map
Influenza A H9N2 (A/brambling/Beijing/16/2012) polymerase Pb1 (Codon Optimized) ORF mammalian expression plasmid, N-His tag
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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