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Influenza A H3N2 (A/Victoria/3/1975) Hemagglutinin / HA ORF mammalian expression plasmid (Codon Optimized)

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H3N2 HA cDNA Clone Product Information
NCBI RefSeq:
RefSeq ORF Size:1704bp
cDNA Description:Full length Clone DNA of Influenza A H3N2 (A/Victoria/3/1975) Hemagglutinin.
Gene Synonym:Hemagglutinin, HA
Species:H3N2
Vector:pGEM-T Vector
Plasmid:pGEM-H3N2-VI-75-HA
Restriction Site:
Tag Sequence:
Sequence Description:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with AFM71967.1.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Influenza A H3N2 (A/Victoria/3/1975) Hemagglutinin / HA ORF mammalian expression plasmid (Codon Optimized) on other vectors
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Influenza A H3N2 (A/Victoria/3/1975) Hemagglutinin / HA (Codon Optimized) ORF mammalian expression plasmid, C-HA tagVG40396-CY$315
Influenza A H3N2 (A/Victoria/3/1975) Hemagglutinin / HA ORF mammalian expression plasmid (Codon Optimized)VG40396-G$115
Influenza A H3N2 (A/Victoria/3/1975) Hemagglutinin / HA (Codon Optimized) ORF mammalian expression plasmid, N-Flag tagVG40396-NF$315
Influenza A H3N2 (A/Victoria/3/1975) Hemagglutinin / HA (Codon Optimized) ORF mammalian expression plasmid, N-His tagVG40396-NH$315
Influenza A H3N2 (A/Victoria/3/1975) Hemagglutinin / HA (Codon Optimized) ORF mammalian expression plasmid, N-Myc tagVG40396-NM$315
Influenza A H3N2 (A/Victoria/3/1975) Hemagglutinin / HA (Codon Optimized) ORF mammalian expression plasmid, N-HA tagVG40396-NY$315
Influenza A H3N2 (A/Victoria/3/1975) Hemagglutinin / HA ORF mammalian expression plasmid (Codon Optimized)VG40396-UT$315
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Background

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA0, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA0) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

References
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). "Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). "Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential". Avian Dis. 40 (2): 425–37
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