Anti-IkB alpha Antibody

Cat: 12045-MM02
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Anti-IkB alpha Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-IkB alpha Antibody
Validated applications
Species reactivity
Reacts with: Human
Human IkB alpha
Recombinant Human IkB alpha / NFKBIA protein (Catalog#12045-H07E)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IkB alpha / NFKBIA (rh IkB alpha / NFKBIA; Catalog#12045-H07E; NP_065390.1; Phe 2-Leu 317). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG2b Clone #02
Protein A
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-IkB alpha Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
ICC-IF 1:50-1:1000  
FCM 1:25-1:100  
ELISA(Det) 1:1000-1:10000 This antibody will detect Human IkB alpha / NFKBIA in ELISA pair set (Catalog: # SEK12045). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 12045-R056).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-IkB alpha Antibody (Mouse Monoclonal antibody) Images
Human IkB alpha/NFKBIA Immunofluorescence(IF) 2879
Immunofluorescence staining of human NFKBIA in HeLa cells with mouse monoclonal antibody (1:60). The image showing nucleus staining of HeLa cells.
Human IkB alpha/NFKBIA Flow Cytometry (FC) 15463
Flow cytometric analysis of Human NFKBIA expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human NFKBIA, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Anti-IkB alpha Antibody Alternative Names
Anti-IKBA Antibody;Anti-MAD-3 Antibody;Anti-NFKBI Antibody
IkB alpha Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IkB alpha, NFKBIA, or IKBA), is a member of the NF-kappa-B inhibitor family that function to inhibit the NF-kB transcription factor. NFKBIA inhibits NF-kB by masking the nuclear localization signals (NLS) of NF-kB proteins and keeping them sequestered in an inactive state in the cytoplasm. In addition, NFKBIA blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-kB's proper functioning. Signal-induced degradation of I kappa B alpha exposes the nuclear localization signal of NF-kappa B, thus allowing it to translocate into the nucleus and activate transcription from responsive genes. An autoregulatory loop is established when NF-kappa B induces expression of the I kappa B alpha gene and newly synthesized I kappa B alpha accumulates in the nucleus where it negatively regulates NF-kappa B-dependent transcription. As part of this post-induction repression, the nuclear export signal on I kappa B alpha mediates transport of NF-kappa B-I kappa B alpha complexes from the nucleus to the cytoplasm. Deletion of NFKBIA has an effect that is similar to the effect of EGFR amplification in the pathogenesis of glioblastoma and is associated with comparatively short survival. Polymorphisms in NFKBIA may be important in pre-disposition to and outcome after treatment, of multiple myeloma (MM). The NFKBIA gene product, IkappaBalpha, binds to NF-kappaB preventing its activation and is important in mediating resistance to apoptosis in B-cell lymphoproliferative diseases.

Full Name
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Research Areas
  • Verma IM, et al. (1995) Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation. Genes Dev. 9 (22): 2723-35.
  • Jacobs MD, et al. (1998) Structure of an IkappaBalpha/NF-kappaB complex. Cell 95 (6): 749-58.
  • Hay RT, et al. (1999) Control of NF-kappa B transcriptional activation by signal induced proteolysis of I kappa B alpha. Philos Trans R Soc Lond B Biol Sci. 354(1389): 1601-9.
  • Spink CF, et al. (2007) Haplotypic structure across the I kappa B alpha gene (NFKBIA) and association with multiple myeloma. Cancer Lett. 246(1-2): 92-9.
  • Bredel M, et al. (2011) NFKBIA deletion in glioblastomas. N Engl J Med. 364(7): 627-37.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-IkB alpha Antibody Mouse Monoclonal ELISA 12045-MM03
    Anti-IkB alpha Antibody (FITC) Mouse Monoclonal FCM,ICC/IF,IF 12045-MM02-F
    Anti-IkB alpha Antibody Rabbit Monoclonal ELISA(Cap) 12045-R056
    Anti-IkB alpha Antibody Rabbit Polyclonal WB,ELISA,IP 12045-RP02
    Anti-IkB alpha Antibody Rabbit Monoclonal WB,ELISA,IP 12045-R116
    Anti-IkB alpha Antibody Rabbit Polyclonal ELISA 12045-RP01
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