Anti-IkB alpha Antibody (FITC)

Cat: 12045-MM02-F
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Anti-IkB alpha Antibody (FITC) (Mouse Monoclonal antibody) General Information
Product name
Anti-IkB alpha Antibody (FITC)
Validated applications
FCM,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human IkB alpha
Immunogen
Recombinant Human IkB alpha / NFKBIA Protein (Catalog#12045-H07E)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IkB alpha / NFKBIA (rh IkB alpha / NFKBIA; Catalog#12045-H07E; NP_065390.1; Phe 2-Leu 317) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Source
Monoclonal Mouse IgG2b Clone #02
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Conjugate
FITC
Concentration
10 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Anti-IkB alpha Antibody (FITC) (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ICC-IF 5-20 μg/mL  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-IkB alpha Antibody (FITC) (Mouse Monoclonal antibody) Images
Human IkB alpha/NFKBIA Flow Cytometry (FC) 2670
NFKBIA expression in HeLa and Jurkat cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and then stained with FITC Mouse anti-NFKBIA. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Human IkB alpha/NFKBIA Immunofluorescence(IF) 2880
Immunofluorescence staining of human NFKBIA in HeLa cells with mouse monoclonal antibody (15 µg/mL). Primary antibody (FITC conjugated) was incubated for 1 hour at 37℃. The image showing nucleus staining of HeLa cells.
Anti-IkB alpha Antibody (FITC) Alternative Names
Anti-IKBA Antibody;Anti-MAD-3 Antibody;Anti-NFKBI Antibody
IkB alpha Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IkB alpha, NFKBIA, or IKBA), is a member of the NF-kappa-B inhibitor family that function to inhibit the NF-kB transcription factor. NFKBIA inhibits NF-kB by masking the nuclear localization signals (NLS) of NF-kB proteins and keeping them sequestered in an inactive state in the cytoplasm. In addition, NFKBIA blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-kB's proper functioning. Signal-induced degradation of I kappa B alpha exposes the nuclear localization signal of NF-kappa B, thus allowing it to translocate into the nucleus and activate transcription from responsive genes. An autoregulatory loop is established when NF-kappa B induces expression of the I kappa B alpha gene and newly synthesized I kappa B alpha accumulates in the nucleus where it negatively regulates NF-kappa B-dependent transcription. As part of this post-induction repression, the nuclear export signal on I kappa B alpha mediates transport of NF-kappa B-I kappa B alpha complexes from the nucleus to the cytoplasm. Deletion of NFKBIA has an effect that is similar to the effect of EGFR amplification in the pathogenesis of glioblastoma and is associated with comparatively short survival. Polymorphisms in NFKBIA may be important in pre-disposition to and outcome after treatment, of multiple myeloma (MM). The NFKBIA gene product, IkappaBalpha, binds to NF-kappaB preventing its activation and is important in mediating resistance to apoptosis in B-cell lymphoproliferative diseases.

Full Name
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Research Areas
References
  • Verma IM, et al. (1995) Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation. Genes Dev. 9 (22): 2723-35.
  • Jacobs MD, et al. (1998) Structure of an IkappaBalpha/NF-kappaB complex. Cell 95 (6): 749-58.
  • Hay RT, et al. (1999) Control of NF-kappa B transcriptional activation by signal induced proteolysis of I kappa B alpha. Philos Trans R Soc Lond B Biol Sci. 354(1389): 1601-9.
  • Spink CF, et al. (2007) Haplotypic structure across the I kappa B alpha gene (NFKBIA) and association with multiple myeloma. Cancer Lett. 246(1-2): 92-9.
  • Bredel M, et al. (2011) NFKBIA deletion in glioblastomas. N Engl J Med. 364(7): 627-37.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-IkB alpha Antibody Mouse Monoclonal ELISA 12045-MM03
    Anti-IkB alpha Antibody Mouse Monoclonal ELISA,ELISA(Det),FCM,ICC/IF,IF 12045-MM02
    Anti-IkB alpha Antibody Rabbit Monoclonal ELISA(Cap) 12045-R056
    Anti-IkB alpha Antibody Rabbit Polyclonal WB,ELISA,IP 12045-RP02
    Anti-IkB alpha Antibody Rabbit Monoclonal WB,ELISA,IP 12045-R116
    Anti-IkB alpha Antibody Rabbit Polyclonal ELISA 12045-RP01
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