Anti-IkB alpha Antibody

Cat: 12045-R116
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Anti-IkB alpha Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-IkB alpha Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human IkB alpha
Immunogen
Recombinant Human IkB alpha / NFKBIA Protein (Catalog#12045-H07E)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human IkB alpha / NFKBIA (rh IkB alpha / NFKBIA; Catalog#12045-H07E; Phe 2-Leu 31; NP_065390.1) .
Source
Monoclonal Rabbit IgG Clone #116
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-IkB alpha Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-IkB alpha Antibody (Rabbit Monoclonal antibody) Images
Human IkB alpha/NFKBIA Western blot (WB) 13460

Anti-NFKBIA rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:37 kDa

Observed band size:38 kDa

Human IkB alpha/NFKBIA Immunoprecipitation(IP) 17696

NFKBIA was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

Lane C:0.5 mg A431 Whole Cell Lysate

Lane D:0.5 mg Hela Whole Cell Lysate

Lane E:0.5 mg K562 Whole Cell Lysate

2 µL anti-NFKBIA rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-NFKBIA rabbit monoclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 36 kDa

Observed band size: 36 kDa

Anti-IkB alpha Antibody Alternative Names
Anti-IKBA Antibody;Anti-MAD-3 Antibody;Anti-NFKBI Antibody
IkB alpha Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IkB alpha, NFKBIA, or IKBA), is a member of the NF-kappa-B inhibitor family that function to inhibit the NF-kB transcription factor. NFKBIA inhibits NF-kB by masking the nuclear localization signals (NLS) of NF-kB proteins and keeping them sequestered in an inactive state in the cytoplasm. In addition, NFKBIA blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-kB's proper functioning. Signal-induced degradation of I kappa B alpha exposes the nuclear localization signal of NF-kappa B, thus allowing it to translocate into the nucleus and activate transcription from responsive genes. An autoregulatory loop is established when NF-kappa B induces expression of the I kappa B alpha gene and newly synthesized I kappa B alpha accumulates in the nucleus where it negatively regulates NF-kappa B-dependent transcription. As part of this post-induction repression, the nuclear export signal on I kappa B alpha mediates transport of NF-kappa B-I kappa B alpha complexes from the nucleus to the cytoplasm. Deletion of NFKBIA has an effect that is similar to the effect of EGFR amplification in the pathogenesis of glioblastoma and is associated with comparatively short survival. Polymorphisms in NFKBIA may be important in pre-disposition to and outcome after treatment, of multiple myeloma (MM). The NFKBIA gene product, IkappaBalpha, binds to NF-kappaB preventing its activation and is important in mediating resistance to apoptosis in B-cell lymphoproliferative diseases.

Full Name
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Research Areas
References
  • Verma IM, et al. (1995) Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation. Genes Dev. 9 (22): 2723-35.
  • Jacobs MD, et al. (1998) Structure of an IkappaBalpha/NF-kappaB complex. Cell 95 (6): 749-58.
  • Hay RT, et al. (1999) Control of NF-kappa B transcriptional activation by signal induced proteolysis of I kappa B alpha. Philos Trans R Soc Lond B Biol Sci. 354(1389): 1601-9.
  • Spink CF, et al. (2007) Haplotypic structure across the I kappa B alpha gene (NFKBIA) and association with multiple myeloma. Cancer Lett. 246(1-2): 92-9.
  • Bredel M, et al. (2011) NFKBIA deletion in glioblastomas. N Engl J Med. 364(7): 627-37.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-IkB alpha Antibody Mouse Monoclonal ELISA 12045-MM03
    Anti-IkB alpha Antibody Mouse Monoclonal ELISA,ELISA(Det),FCM,ICC/IF,IF 12045-MM02
    Anti-IkB alpha Antibody (FITC) Mouse Monoclonal FCM,ICC/IF,IF 12045-MM02-F
    Anti-IkB alpha Antibody Rabbit Monoclonal ELISA(Cap) 12045-R056
    Anti-IkB alpha Antibody Rabbit Polyclonal WB,ELISA,IP 12045-RP02
    Anti-IkB alpha Antibody Rabbit Polyclonal ELISA 12045-RP01
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