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Izumo sperm-egg fusion 1 Protein Datasheet
IZUMO1 Protein Price Inquiry ( Available Sizes )
IZUMO1 Protein Product Information
A DNA sequence encoding the human IZUMO1 (AAH34769.1) (Met1-Arg292) was expressed with a polyhistidine tag at the C-terminus.
|Expression Host:||Human Cells|
IZUMO1 Protein QC Testing
|Purity:||> 95% as determined by SDS-PAGE||SDS-PAGE:
|Endotoxin:||< 1.0 EU per μg of the protein as determined by the LAL method|
|Stability:||Samples are stable for up to twelve months from date of receipt at -70℃|
|Predicted N terminal:||Cys 22|
The recombinant human IZUMO1 consists of 282 amino acids and predicts a molecular mass of 32.1 KDa. It migrates as an approximately 43 KDa band in SDS-PAGE under reducing conditions.
|Formulation:||Lyophilized from sterile PBS, pH 7.4.
IZUMO1 Protein Usage Guide
|Storage:||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution:||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
IZUMO1 Protein Related Products & Topics
IZUMO1 Protein Description
IZUMO1 has an N-terminal domain with significant homology to the N-terminal domain of Izumo. Izumo is a sperm membrane protein which plays a key role in the fusion in the mouse. It has an Immunoglobulin (Ig) domain and an N-terminal domain for which neither the functions nor homologous sequences are known. Besides IZUMO1, there are other 3 proteins which have an N-terminal domain with significant homology to the N-terminal domain of Izumo. They are Izumo 2, 3, and 4. Izumo 1-3 are transmembrane proteins expressed specifically in the testis, and Izumo 4 is a soluble protein expressed in the testis and in other tissues. Izumo 1, 3, and 4 formed protein complexes on sperm, Izumo 1 forming several larger complexes and is involved in the fertilization process. It also may be involved in the fusion of the sperm with the egg.
- Inoue N. et al., 2008, Biochem Biophys Res Commun. 377 (3): 910-4.
- Ikram MK. et al., 2010, PLoS Genet. 6 (10): e1001184.
- Sklar P. et al., 2011, Nat Genet. 43 (10): 977-83.