IZUMO1 Protein (His Tag)

Izumo sperm-egg fusion 1 Protein Datasheet

IZUMO1 Protein Product Information

• Synonym: IZUMO1
• Protein Construction: A DNA sequence encoding the human IZUMO1 (AAH34769.1) (Met1-Arg292) was expressed with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

IZUMO1 Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Cys 22
• Molecular Mass: The recombinant human IZUMO1 consists of 282 amino acids and predicts a molecular mass of 32.1 KDa. It migrates as an approximately 43 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IZUMO1 protein

IZUMO1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IZUMO1 Protein Description

IZUMO1 has an N-terminal domain with significant homology to the N-terminal domain of Izumo. Izumo is a sperm membrane protein which plays a key role in the fusion in the mouse. It has an Immunoglobulin (Ig) domain and an N-terminal domain for which neither the functions nor homologous sequences are known. Besides IZUMO1, there are other 3 proteins which have an N-terminal domain with significant homology to the N-terminal domain of Izumo. They are Izumo 2, 3, and 4. Izumo 1-3 are transmembrane proteins expressed specifically in the testis, and Izumo 4 is a soluble protein expressed in the testis and in other tissues. Izumo 1, 3, and 4 formed protein complexes on sperm, Izumo 1 forming several larger complexes and is involved in the fertilization process. It also may be involved in the fusion of the sperm with the egg.

References

1. Inoue N. et al., 2008, Biochem Biophys Res Commun. 377 (3): 910-4.
2. Ikram MK. et al., 2010, PLoS Genet. 6 (10): e1001184.
3. Sklar P. et al., 2011, Nat Genet. 43 (10): 977-83.