Cynomolgus monkey ITGB1 Gene cDNA Clone / ORF Clone

ITGB1 cDNA Clone Datasheet

ITGB1 cDNA Clone Product Information

• cDNA Size: 2397
• Gene Synonym: ITGB1
• Source: Cynomolgus monkey

ITGB1 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 338 A/G resulting in the amino acid Gln substitution by Arg and 1464 G/A not causing the amino acid variation.
  Please check the sequence information before order.

ITGB1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ITGB1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ITGB1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAATTTAC AACTGATTTT CTGGATTGGA CTGATCAGTT CAATTTGCTG TGTGTTTGGT
61       CAAACAGATG AAAATAGATG TTTAAAAGCA AATGCCAAAT CATGTGGAGA ATGTATACAA
121      GCAGGGCCAA ATTGTGGGTG GTGCACAAAT TCAACATTTT TACAGGAAGG AATGCCCACT
181      TCTGCACGAT GTGATGATTT AGAAGCCTTA AAAAAGAAGG GTTGCCCTCC AGATGACATA
241      GAAAATCCCA GAGGCTCCAA AGATATAAAG AAAAATAAAA ATGTAACCAA CCGTAGCAAA
301      GGAACAGCAG AGAAGCTCAA GCCAGAGGAT ATTACTCGGA TCCAACCACA GCAGTTGGTT
361      TTGCGATTAA GATCAGGGGA GCCACAGACA TTTACATTAA AATTCAAGAG AGCTGAAGAC
421      TATCCCATTG ACCTCTACTA CCTTATGGAC CTGTCTTACT CAATGAAAGA CGATTTGGAG
481      AATGTAAAAA GTCTTGGAAC AGATCTGATG AATGAAATGA GGAGGATTAC GTCTGACTTC
541      AGAATTGGAT TTGGTTCATT TGTGGAAAAG ACTGTGATGC CTTACATTAG CACAACACCA
601      GCTAAGCTCA GGAACCCTTG CACAAGTGAA CAGAACTGCA CCAGCCCATT TAGCTACAAA
661      AATGTGCTCA GTCTTACTAA TAAAGGAGAA GTATTTAATG AACTTGTTGG AAAACAGCGC
721      ATATCTGGAA ATTTGGATTC TCCAGAAGGT GGTTTTGATG CCATCATGCA AGTTGCAGTT
781      TGTGGATCAC TGATTGGCTG GAGGAATGTT ACACGGCTGC TGGTGTTTTC CACAGATGCC
841      GGGTTTCACT TTGCTGGAGA TGGGAAACTT GGTGGCATTG TTTTACCAAA TGATGGACAG
901      TGTCACCTGG AAAATAATAT GTACACAATG AGTCATTATT ATGATTATCC TTCTATTGCT
961      CACCTTGTCC AGAAACTGAG TGAAAATAAT ATTCAGACAA TTTTTGCAGT TACTGAAGAA
1021     TTTCAGCCCG TTTACAAGGA ACTGAAAAAC TTGATCCCTA AGTCAGCAGT AGGAACATTA
1081     TCTGCAAATT CTAGTAATGT AATTCAGTTG ATCATTGATG CATACAATTC CCTTTCCTCA
1141     GAAGTCATTT TGGAAAACGG CAAATTGTCA GAAGGAGTAA CAATAAGTTA CAAATCTTAC
1201     TGCAAGAACG GGGTGAATGG AACAGGGGAA AATGGAAGAA AATGTTCCAA TATTTCCATT
1261     GGAGATGAGG TTCAATTTGA AATTAGCATA ACTTCAAATA AGTGTCCAAA AAAGGATTCT
1321     GACAGCTTTA AAATTAGGCC TCTGGGCTTC ACGGAGGAAG TAGAGGTTAT TCTTCAGTAC
1381     ATCTGTGAAT GTGAATGCCA AAGCGAAGGC ATCCCTGAAA GTCCCAAGTG TCATGAAGGA
1441     AATGGGACGT TTGAGTGTGG CGCATGCAGG TGCAACGAAG GGCGTGTTGG TAGACATTGT
1501     GAATGCAGCA CAGATGAAGT TAACAGTGAA GACATGGACG CTTACTGCAG AAAAGAAAAC
1561     AGTTCAGAAA TCTGCAGTAA CAATGGAGAG TGCGTTTGTG GACAGTGTGT TTGTAGGAAG
1621     AGGGATAATA CAAATGAAAT TTATTCTGGC AAATTCTGCG AGTGTGATAA TTTCAACTGT
1681     GATAGATCCA ATGGCTTAAT TTGTGGAGGA AATGGTGTTT GCAAGTGTCG TGTGTGTGAG
1741     TGCAACCCCA ACTACACTGG CAGTGCATGT GACTGTTCTT TGGATACTAG TACTTGCGAA
1801     GCCAGCAACG GACAGATCTG TAATGGCCGG GGCATCTGCG AATGTGGTGT CTGTAAGTGT
1861     ACAGATCCGA AGTTCCAAGG GCAAACATGT GAGATGTGTC AGACCTGCCT TGGTGTCTGT
1921     GCTGAGCATA AAGAATGTGT TCAGTGCAGA GCCTTCAATA AAGGAGAAAA GAAAGACACA
1981     TGCGCACAGG AATGTTCCTA TTTTAACATT ACCAAGGTAG AAAGTCGGGA CAAATTACCC
2041     CAGCCAGTCC AACCTGATCC TGTGTCCCAT TGTAAGGAGA AGGATGTTGA CGACTGTTGG
2101     TTCTATTTTA CATATTCAGT GAATGGGAAC AATGAGGTCA TGGTTCATGT TGTGGAGAAT
2161     CCAGAGTGTC CCACTGGTCC AGACATCATT CCAATTGTAG CTGGTGTGGT TGCTGGAATT
2221     GTTCTTATTG GCCTTGCATT ACTGCTGATA TGGAAGCTTT TAATGATAAT TCATGACAGA
2281     AGGGAGTTTG CTAAATTTGA AAAGGAGAAA ATGAATGCCA AATGGGACAC AGGTGAAAAT
2341     CCTATTTATA AGAGTGCCGT GACAACTGTG GTCAATCCGA AGTATGAGGG AAAATGA

 

Quality Control:cDNA sequencing results is available upon request.