Cynomolgus monkey ITGAV Gene cDNA Clone / ORF Clone

ITGAV cDNA Clone Datasheet

ITGAV cDNA Clone Product Information

• cDNA Size: 3147
• Gene Synonym: ITGAV
• Source: Cynomolgus monkey

ITGAV cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 44 G/A resulting in the amino acid Gly substitution by Asp and 279 T/A, 1503 T/C, 1710 G/A, 2403 C/T not causing the amino acid variation.
  Please check the sequence information before order.

ITGAV cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ITGAV cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ITGAV cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTTCTC CGCCGCGGCG ACGGCTGCGC CTCGGTCCCC GCGACCTCCC GCTTCTTCTC
61       TCGGGACTCC TGCTACCTCT GTGCCGCGCC TTCAACCTAG ACGTGGACAG TCCTGCCGAG
121      TACTCTGGCC CCGAGGGAAG TTACTTCGGC TTCGCCGTGG ATTTCTTCGT GCCCAGCGCG
181      TCTTCCCGCA TGTTTCTTCT CGTGGGAGCT CCCAAAGCAA ACACGACCCA GCCTGGGATT
241      GTGGAAGGAG GGCAAGTCCT CAAATGTGAC TGGTCTTCAA CCCGCCGGTG CCAGCCAATT
301      GAATTTGATG CAACAGGCAA TAGAGATTAT GCCAAGGATG ATCCATTGGA ATTTAAGTCC
361      CACCAGTGGT TTGGAGCATC TGTGAGGTCG AAACAAGATA AAATTTTGGC CTGTGCCCCA
421      TTGTACCATT GGAGGACTGA GTTGAAACAG GAGCGAGAGC CTGTTGGAAC ATGCTTTCTT
481      CAAGATGGAA CAAAGACTGT TGAGTATGCT CCATGCAGAT CACAAGATAT TGATGCTGAT
541      GGACAGGGAT TTTGTCAAGG AGGATTCAGC ATTGATTTTA CTAAAGCTGA CAGAGTACTT
601      CTTGGTGGTC CTGGTAGCTT TTATTGGCAA GGTCAGCTTA TTTCGGATCA AGTGGCAGAA
661      ATCGTATCTA AATATGACCC CAATGTTTAC AGCATCAAGT ATAATAACCA ATTAGCAACT
721      CGGACTGCAC AAGCTATTTT TGATGACAGT TATTTGGGTT ACTCTGTGGC TGTCGGAGAT
781      TTCAATGGTG ATGGCATAGA TGACTTTGTT TCAGGAGTTC CAAGAGCAGC AAGGACTTTG
841      GGAATGGTTT ATATTTATGA TGGGAAGAAC ATGTCCTCCA TATACAATTT TACTGGCGAT
901      CAGATGGCTG CATATTTCGG GTTTTCTGTA GCTGCCACTG ACATTAATGG AGATGATTAT
961      GCAGATGTGT TTATTGGAGC ACCTCTCTTC ATGGATCGTG GCTCTGATGG CAAACTCCAG
1021     GAGGTGGGGC AGGTCTCAGT GTCTCTACAG AGAGCTTCAG GAGACTTCCA GACAACAAAG
1081     CTGAATGGGT TTGAGGTCTT TGCACGGTTT GGCAGTGCCA TCGCTCCTTT GGGAGATCTG
1141     GACCAGGATG GTTTCAATGA TATTGCAATT GCTGCTCCAT ATGGGGGTGA AGATAAAAAA
1201     GGAATTGTTT ATATCTTCAA TGGAAGATCA ACTGGCTTGA ACGCAGTCCC ATCTCAAATC
1261     CTTGAAGGGC AGTGGGCTGC TCGAAGCATG CCACCAAGCT TTGGCTATTC AATGAAAGGA
1321     GCCACAGATA TAGACAAAAA TGGATATCCA GACTTAATTG TAGGAGCTTT TGGTGTAGAT
1381     CGAGCTATCT TATACAGGGC CCGACCAGTT ATCACTGTAA ATGCTGGTCT TGAAGTGTAC
1441     CCTAGCATTT TAAATCAAGA CAATAAAACC TGCTCACTGC CTGGAACAGC TCTCAAAGTT
1501     TCCTGCTTTA ATGTTAGGTT CTGCTTAAAG GCAGATGGCA AAGGAGTACT TCCCAGAAAG
1561     CTTAATTTCC AGGTGGAACT TCTTTTGGAT AAACTCAAGC AAAAGGGAGC AATTCGACGA
1621     GCACTGTTTC TCTACAGCAG GTCCCCAAGT CACTCCAAGA ACATGACTAT TTCAAGGGGG
1681     GGACTGATGC AGTGTGAGGA ATTGATAGCA TATCTGCGGG ATGAATCTGA ATTTAGAGAC
1741     AAACTCACTC CAATTACCAT TTTTATGGAA TATTGGTTGG ATTATAGAAC AGCTGCTGAT
1801     ACAACAGGCT TGCAACCCAT TCTTAACCAG TTCACGCCTG CTAACATTAG TCGACAGGCT
1861     CACATTCTAC TTGACTGTGG TGAAGACAAT GTCTGTAAAC CCAAACTGGA AGTTTTTGTA
1921     GATAGTGATC AAAAGAAGAT CTATATTGGG GATGACAACC CTCTGACATT GATTGTTAAG
1981     GCTCAGAATC AAGGAGAAGG TGCCTATGAA GCTGAGCTCA TCGTTTCCAT TCCACTGCAG
2041     GCTGATTTCA TCGGGGTTGT CCGAAACAGT GAAGCCTTAG CAAGACTTTC CTGTGCATTT
2101     AAGACAGAAA ACCAAACTCG CCAGGTGGTA TGTGACCTTG GAAACCCAAT GAAGGCTGGA
2161     ACTCAACTCT TAGCTGGTCT TCGTTTCAGT GTGCACCAGC AGTCAGAGAT GGATACTTCT
2221     GTGAAATTTG ACTTACAAAT CCAAAGCTCA AATCTGTTTG ACAAAGTAAG CCCGGTTGTA
2281     TCTCACAAAG TTGACCTTGC TGTTTTAGCT GCAGTTGAGA TAAGAGGAGT CTCGAGTCCT
2341     GATCATATCT TTCTTCCGAT TCCAAACTGG GAGCACAAGG AGAACCCTGA GACTGAAGAA
2401     GATGTTGGGC CAGTTGTTCA GCACATCTAT GAGCTGAGAA ACAATGGTCC AAGTTCATTC
2461     AGCAAGGCAA TGCTCCATCT TCAGTGGCCT TATAAATACA ACAATAATAC TCTGTTGTAT
2521     ATCCTTCATT ATGATATTGA TGGACCAATG AACTGCACTT CAGATATGGA GATCAACCCT
2581     TTGAGAATTA AGATTTCATC TTTGCAAGCA ACTGAAAAGA ATGACACAGT TGCTGGGCAA
2641     GGTGAGCGGG ACCATCTCAT CACTAAGCGG GATCTTGCCC TCAGTGAAGG AGATATTCAC
2701     ACTTTGGGTT GTGGAGTCGC TCAGTGCTTG AAGATTGTCT GCCAAGTTGG AAGATTAGAC
2761     AGAGGAAAGA GTGCAATCTT GTACGTAAAG TCATTACTGT GGACTGAGAC CTTTATGAAT
2821     AAGGAAAATC AGAATCATTC CTATTCTCTG AAGTCATCTG CTTCATTTAA TGTCATAGAG
2881     TTTCCTTATA AAAATCTTCC AATTGAGGAT ATCACCAACT CCACATTGGT TACCACTAAT
2941     GTCACCTGGG GCATTCAGCC AGCACCCATG CCTGTGCCTG TGTGGGTGAT CATTTTAGCA
3001     GTTCTTGCAG GATTGTTGCT ACTGGCTGTT TTGGTATTTG TAATGTACAG GATGGGCTTT
3061     TTTAAACGGG TCCGGCCACC TCAAGAGG

 

Quality Control:cDNA sequencing results is available upon request.