Rat ITGAD Gene cDNA Clone / ORF Clone

ITGAD cDNA Clone Datasheet

ITGAD cDNA Clone Product Information

• Gene Bank Ref.ID: NM_031691.1
• cDNA Size: 3487
• Gene Synonym: Itgax; Itgad
• Source: Rat

ITGAD cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus integrin, alpha D DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 709 A/G, 1487 T/G, 2036 C/T, 2243 A/G, 2969 G/A, 3098 A/G, 3314 C/T resulting in the amino acid Met substitution by Leu, Phe substitution by Cys, Ala substitution by Val, His substitution by Arg, Gly substitution by Asp, Asp substitution by Gly, Ala substitution by Val.

ITGAD cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ITGAD cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ITGAD cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCGGTG GAGTTGTGAT CCTCCTGTGT GGCTGGGTCC TGGCTTCCTG TCATGGGTCT
61       AACCTGGATG TGGAGGAACC CATCGTGTTC AGAGAGGATG CAGCCAGCTT TGGACAGACT
121      GTGGTGCAGT TTGGTGGATC TCGACTCGTG GTGGGAGCCC CTCTGGAGGC GGTGGCAGTC
181      AACCAAACAG GACGGTTGTA TGACTGTGCA CCTGCCACTG GCATGTGCCA GCCCATCGTA
241      CTGCGCAGTC CCCTAGAGGC AGTGAACATG TCCCTGGGCC TGTCTCTGGT GACTGCCACC
301      AATAACGCCC AGTTGCTGGC TTGTGGTCCA ACTGCACAGA GAGCTTGTGT GAAGAACATG
361      TATGCGAAAG GTTCCTGCCT CCTTCTCGGC TCCAGCTTGC AGTTCATCCA GGCAGTCCCT
421      GCCTCCATGC CAGAGTGTCC AAGACAAGAG ATGGACATTG CTTTCCTGAT TGATGGTTCT
481      GGCAGCATTA ACCAAAGGGA CTTTGCCCAG ATGAAGGACT TTGTCAAAGC TTTGATGGGA
541      GAGTTTGCGA GCACCAGCAC CTTGTTCTCC CTGATGCAAT ACTCGAACAT CCTGAAGACC
601      CATTTTACCT TCACTGAATT CAAGAACATC CTGGACCCTC AGAGCCTGGT GGATCCCATT
661      GTCCAGCTGC AAGGCCTGAC CTACACAGCC ACAGGCATCC GGACAGTGGT GGAAGAGCTA
721      TTTCATAGCA AGAATGGGTC CCGTAAAAGT GCCAAGAAGA TCCTCCTTGT CATCACAGAT
781      GGGCAGAAAT ACAGAGACCC CCTGGAGTAT AGTGATGTCA TTCCCGCCGC AGACAAAGCT
841      GGCATCATTC GTTATGCTAT TGGGGTGGGA GATGCCTTCC AGGAGCCCAC TGCCCTGAAG
901      GAGCTGAACA CCATTGGCTC AGCTCCCCCA CAGGACCACG TGTTCAAGGT AGGCAACTTT
961      GCAGCACTTC GCAGCATCCA GAGGCAACTT CAGGAGAAAA TCTTCGCCAT TGAGGGAACT
1021     CAATCAAGGT CAAGTAGTTC CTTTCAGCAC GAGATGTCAC AAGAAGGTTT CAGTTCAGCT
1081     CTCACATCGG ATGGACCCGT TCTGGGGGCC GTGGGAAGCT TCAGCTGGTC CGGAGGTGCC
1141     TTCTTATATC CCCCAAATAC GAGACCCACC TTTATCAACA TGTCTCAGGA GAATGTGGAC
1201     ATGAGAGACT CCTACCTGGG TTACTCCACC GCAGTGGCCT TTTGGAAGGG GGTTCACAGC
1261     CTGATCCTGG GGGCCCCGCG TCACCAGCAC ACGGGGAAGG TTGTCATCTT TACCCAGGAA
1321     GCCAGGCATT GGAGGCCCAA GTCTGAAGTC AGAGGGACAC AGATCGGCTC CTACTTCGGG
1381     GCCTCTCTCT GTTCTGTGGA CGTGGATAGA GATGGCAGCA CYGACCTGGT CCTGATCGGA
1441     GCCCCCCATT ACTATGAGCA GACCCGAGGG GGGCAGGTCT CAGTGTGCCC CGTGCCCGGT
1501     GTGAGGGGCA GGTGGCAGTG TGAGGCCACC CTCCACGGGG AGCAGGGCCA TCCTTGGGGC
1561     CGCTTTGGGG TGGCTCTGAC AGTGCTGGGG GACGTAAACG GGGACAATCT GGCAGACGTG
1621     GCTATTGGTG CCCCTGGAGA GGAGGAGAGC AGAGGTGCTG TCTACATATT TCATGGAGCC
1681     TCGAGACTGG AGATCATGCC CTCACCCAGC CAGCGGGTCA CTGGCTCCCA GCTCTCCCTG
1741     AGACTGCAGT ATTTTGGGCA GTCATTGAGT GGGGGTCAGG ACCTTACACA GGATGGCCTG
1801     GTGGACCTGG CCGTGGGAGC CCAGGGGCAC GTACTGCTGC TCAGGAGTCT GCCTCTGCTG
1861     AAAGTGGAGC TCTCCATAAG ATTCGCCCCC ATGGAGGTGG CAAAGGCTGT GTACCAGTGC
1921     TGGGAAAGGA CTCCCACTGT CCTCGAAGCT GGAGAGGCCA CTGTCTGTCT CACTGTCCAC
1981     AAAGGCTCAC CTGACCTGTT AGGTAATGTC CAAGGCTCTG TCAGGTATGA TCTGGTGTTA
2041     GATCCGGGCC GCCTGATTTC TCGTGCCATT TTTGATGAGA CTAAGAACTG CACTTTGACG
2101     GGAAGGAAGA CTCTGGGGCT TGGTGATCAC TGCGAAACAG TGAAGCTGCT TTTGCCGGAC
2161     TGTGTGGAAG ATGCAGTGAG CCCTATCATC CTGCGCCTCA ACTTTTCCCT GGTGAGAGAC
2221     TCTGCTTCAC CCAGGAACCT GCGTCCTGTG CTGGCTGTGG GCTCACAAGA CCACATAACT
2281     GCTTCTCTGC CGTTTGAGAA GAACTGTAAG CAAGAACTCC TGTGTGAGGG GGACCTGGGC
2341     ATCAGCTTTA ACTTCTCAGG CCTGCAGGTC TTGGTGGTGG GAGGCTCCCC AGAGCTCACT
2401     GTGACAGTCA CTGTGTGGAA TGAGGGTGAG GACAGCTATG GAACTTTAGT CAAGTTCTAC
2461     TACCCAGCAG GGCTATCTTA CCGACGGGTA ACAGGGACTC AGCAACCTCA TCAGTACCCA
2521     CTACGCTTGG CCTGTGAGGC TGAGCCCGCT GCCCAGGAGG ACCTGAGGAG CAGCAGCTGT
2581     AGCATTAATC ACCCCATCTT CCGAGAAGGT GCAAAGACCA CCTTCATGAT CACATTCGAT
2641     GTCTCCTACA AGGCCTTCCT AGGAGACAGG TTGCTTCTGA GGGCCAAAGC CAGCAGTGAG
2701     AATAATAAGC CTGATACCAA CAAGACTGCC TTCCAGCTGG AGCTCCCAGT GAAGTACACC
2761     GTCTATACCC TGATCAGTAG GCAAGAAGAT TCCACCAACC ATGTCAACTT TTCATCTTCC
2821     CACGGGGGGA GAAGGCAAGA AGCCGCACAT CGCTATCGTG TGAATAACCT GAGTCCACTG
2881     AAGCTGGCCG TCAGAGTTAA CTTCTGGGTC CCTGTCCTTC TGAACGGTGT GGCTGTGTGG
2941     GACGTGACTC TGAGCAGCCC AGCACAGGAT GTCTCCTGCG TGTCCCAGAT GAAACCTCCT
3001     CAGAATCCCG ACTTTCTGAC CCAGATTCAG AGACGTTCTG TGCTGGACTG CTCCATTGCT
3061     GACTGCCTGC ACTTCCGCTG TGACATCCCC TCCTTGGGCA TCCAGGATGA ACTTGACTTC
3121     ATTCTGAGGG GCAACCTCAG CTTCGGCTGG GTCAGTCAGA CATTGCAGGA AAAGGTGTTG
3181     CTTGTGAGTG AGGCTGAAAT CACTTTCGAC ACATCTGTGT ACTCCCAGCT GCCAGGACAG
3241     GAGGCATTTC TGAGAGCCCA GGTGGAGACA ACGTTAGAAG AATACGTGGT CTATGAGCCC
3301     ATCTTCCTCG TGGCTGGGCA GCTCGGTGGG AGGTCTGCTG TTACTGGCTC TCATCACAGT
3361     GGTACTGTAC AAGCTTGGCT TCTTCAAACG TCAGTACAAA GAAATGCTGG ACGGCAAGGC
3421     TGCAGATCCT GTCACAGCCG GCCAGGCAGA TTTCGGCTGT GAGACTCCTC CATATCTCGT
3481     GAGCTAG

 

Quality Control:cDNA sequencing results is available upon request.