ITGA5 & ITGB1 Heterodimer Antibody

ITGA5 & ITGB1 Heterodimer Antibody Datasheet

ITGA5 & ITGB1 Heterodimer Antibody Product Information

• Immunogen: Recombinant Human ITGA5 & ITGB1 Heterodimer protein (Catalog#CT014-H2508H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 01
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ITGA5 & ITGB1 Heterodimer (rh ITGA5 & ITGB1 Heterodimer; Catalog#CT014-H2508H; P08648, P05556-1; Met 1-Tyr 995, Met 1-Asp 728). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

ITGA5 & ITGB1 Heterodimer Antibody Usage Guide

• Specificity: Human ITGA5 & ITGB1 Heterodimer
• No cross-reactivity in ELISA with

  Human cell lysate (293 cell line)

• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human ITGA5 & ITGB1 Heterodimer. The detection limit for Human ITGA5 & ITGB1 Heterodimer is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

ITGA5 & ITGB1 Heterodimer Antibody Background

Integrin alpha-5, also known as CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, CD49e and ITGA5, is a single-pass type I membrane protein which belongs to theintegrin alpha chain family. ITGA5 contains 7FG-GAP repeats. Integrin alpha-5 / beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Integrin beta-1, also known as Fibronectin receptor subunit beta, VLA-4 subunit beta, CD29 and ITGB1, is a single-pass type I membrane protein which belongs to theintegrin beta chain family. It contains oneVWFA domain. Integrin beta-1 / ITGB1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha-5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. It binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5. Integrin beta-1 / ITGB1 interacts with FLNA, FLNB and RANBP9. Integrin beta-1 / ITGB1 binds to human echoviruses 1 and 8 capsid proteins and acts as a receptor for these viruses.

Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen.Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Integrin alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1.

References

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