Cynomolgus monkey ITGA4 Gene cDNA Clone / ORF Clone

ITGA4 cDNA Clone Datasheet

ITGA4 cDNA Clone Product Information

• cDNA Size: 3099
• Gene Synonym: ITGA4
• Source: Cynomolgus monkey

ITGA4 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1706 G/A resulting in the amino acid Arg substitution by Gln and 927 T/G not causing the amino acid variation.
  Please check the sequence information before order.

ITGA4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ITGA4 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ITGA4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTTGGG AAGCGAGGCG CGAACCTGGT CCCCGAAGGG CCGCCCTCCG GGAGACGGTG
61       ATGTTGTTGC TGTGCCTGGG GGTCCCGACC GGCCGCCCCT ACAACGTGGA CACTGAGAGC
121      GCGCTGCTTT ACCAGGGCCC CCCCAACACG CTGTTCGGCT ACTCGGTCGT GCTGCACAGC
181      CAAGGGGCGA ACAGATGGCT CGTGGTGGGT GCGCCCACTG CCAACTGGCT CGCCAACGCT
241      TCAGTGATCA ATCCCGGGGC GATTTACAGA TGCAGGATCG GGAAGAATCC CGGCCGGACG
301      TGCGAACAGC TCCAGCTGGG TAGCCCTAAT GGAGAACCTT GTGGAAAGAC TTGTTTGGAA
361      GAGAGAGACA ATCAGTGGTT GGGGGTCACA CTTTCCAGAC AGCCAGGAGA AAATGGATCC
421      ATCGTGACTT GTGGGCATAG ATGGAAAAAT ATATTTTACA TAAAGAATGA AAATAAGCTC
481      CCCACTGGTG GTTGCTATGG AATGCCCCCT GATTTACGAA CAGAACTGAG TAAAAGAATA
541      GCTCCGTGTT ATCAAGATTA TGTGAAAAAA TTTGGAGAAA ATTTTGCATC ATGTCAAGCT
601      GGAATATCTA GTTTTTACAC AAAGGATTTA ATTGTGATGG GAGCCCCAGG ATCATCTTAT
661      TGGACTGGCT CTCTTTTTGT CTACAATATA ACTACAAATA AATACAAGGC TTTTTTAGAC
721      AGACAAAATC AAGTAAAATT TGGAAGTTAT TTAGGATATT CAGTTGGAGC TGGTCATTTT
781      CGGAGTCAGC ATACTACTGA AGTAGTTGGA GGAGCTCCTC AACACGAGCA GATTGGTAAA
841      GCATATATAT TCGGCATTGA TGAAAAAGAA CTAAATATCT TACATGAAAT GAAAGGTAAA
901      AAGCTTGGAT CGTACTTTGG AGCTTCGGTC TGTGCTGTGG ACCTTAACGC AGATGGCTTC
961      TCAGATCTGC TCGTGGGAGC GCCCATGCAG AGCACCATCA GAGAGGAAGG AAGAGTGTTT
1021     GTGTACATCA ACTCCGGCTC GGGAGCAGTA ATGAATGAAA TGGAAACAAA CCTCGTTGGA
1081     AGTGACAAAT ATGCTGCAAG ATTTGGCGAA TGTATAGTTA ATCTTGGTGA CATTGACAAT
1141     GATGGCTTTG AAGATGTTGC TATTGGAGCT CCACAAGAAG ATGACTTGCA AGGTGCTATT
1201     TATATTTACA ATGGCCGTGC AGATGGGGTC TCATCAGCCT TCTCGCAGAG AATCGAAGGA
1261     CTTCAGATCA GCAAATCGTT AAGTATGTTT GGACAGTCTA TATCAGGACA AATTGATGCA
1321     GATAATAATG GATATGTAGA TGTAGCAGTT GGTGCTTTTC GGTCTGATTC TGCTGTGTTG
1381     CTAAGGACAA GACCTGTAGT CATTGTTGAA GCTTCTTTAA GCCACCCTGA GTCAGTAAAT
1441     AGAACGAAAT TTGACTGTGT TGAAAATGGA TGGCCTTCTG TGTGCATGGA TCTAACACTT
1501     TGTTTCTCAT ATAAGGGCAA GGAAGTTCCA GGTTACATTG TTTTGTTTTA TAACATGAGT
1561     TTGGATGTGA ACAGAAAGAC AGAGTCTCCA CCAAGATTCT ATTTTTCTTC TAATGGAACT
1621     TCTGACGTGA TTACAGGAAG CATACAAGTG TCCAGCGGAG TAGCTAACTG TAGAACACAT
1681     CAAGCATTTA TGCGGAAAGA TGTGCAGGAC ATCCTTACCC CAATTCAGAT TGAAGCTGCT
1741     TACCACCTTG GTCCTCACGT CATCAGTAAA CGAAGCACAG AGGAATTCCC ACCACTTCAG
1801     CCAATTCTTC AGCAGAAGAA AGAAAAAGAC ATAATTAAAA AAACAATAAG CTTTGCAAGG
1861     TTTTGTGCCC ATGAAAATTG TTCTGCTGAT TTACAGGTTT CTGCAAAGAT TGGGTTTTTC
1921     AAGCCCCATG AAAATAAAAC CTACCTTGCT GTTGGGAGTA TGAAGACATT AATGTTGAAT
1981     GTGTCCTTGT TTAATGCTGG AGATGATGCA TATGAAACGA CTCTACATGT CAAACTACCC
2041     ATGGGTCTTT ACTTCATTAA GATTTTAGAG CTGGAAGAGA AGCAAATAAA CTGTGAAGTC
2101     ACAGATAACT CTGGCATGGT ACAACTTGAC TGCAGTATTG GCTATATATA TGTAGATCAT
2161     CTCTCAAGGA TAGATATTAG CTTTCTCCTG GATGTGAGCT CACTCAGCAG AGCGGAAGAG
2221     GACCTCAATA TCACAGTGCA TGCTACCTGT GAAAATGAAG GGGAAATGGA CAATCTAAAG
2281     CACAGCAGAG TGACTGTAGC AATACCTTTA AAATATGAGG TTAAGCTGAC TGTTCATGGG
2341     TTTGTAAACC CAACTTCATT TGTGTATGGA TCAAATGATG AAAATGAGCC TGAAATGTGC
2401     ATGGTGGAGA AAATGAACTT AACTTTCCAT GTTATCAACA CTGGCAATAG CATGGCTCCT
2461     AATGTTAGTG TGGAAATAAT GGTACCAAAT TCTTTCAGCC CCCAAACTGA TAAGCTGTTC
2521     AACATTTTGG ATGTCCAGAC TACTACTGGA GAATGCCACT TTGAAAATTA TCAAAGAGTA
2581     TGTGCATTAG AGCAGCAAAA GAGTGCAATG CACACCTTGA AAGGCATGTT CCAGTTCTTG
2641     TCCAAGACTG ATAAGAGGCT ATTGTACTGC ATAAAAGCTG ATCCACATTG TTTAAATTTC
2701     CTATGTAATT TCGGGAAAAT GGAAAGTGGA AAAGAAGCCA GTGTTCATAT CCAGTTGGAA
2761     GGCCGACCAT CCATTTTAGA AATGGATGAG ACTTCAGCAC TCAAATTTGA AATAAGAGCA
2821     ACAAGTTTTC CAGAGCCAAA TTCAAGAGTT ATTGAACTAA ACAAGGATGA GAATGTTGCA
2881     CATGTTCTCC TGGAAGGACT ACATCATCAA AGACCCAAAC GTTATTTCAC CATAGTGATT
2941     ATTTCAAGTA GCTTGCTACT TGGACTTATT GTACTTCTGT TGATCTCATA TGTTATGTGG
3001     AAGGCCGGCT TCTTTAAAAG ACAATACAAA TCTATCCTAC AAGAAGAAAA CAGAAGAGAC
3061     AGTTGGAGTT ACATCAACAG TAAAAGCAAT GATGATTAA

 

Quality Control:cDNA sequencing results is available upon request.