Rat ITGA4 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80291-G	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

ITGA4 cDNA Clone Datasheet

ITGA4 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001107737.1
cDNA Size:	3111
Gene Synonym:	Itga4
Source:	Rat

ITGA4 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus integrin, alpha 4 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 216 T/C, 2121 C/T, 2442 T/C and 2598 A/C not causing the amino acid variation.

ITGA4 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ITGA4 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ITGA4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTGCGG AAGCGATGTG CAGACCGAGG CCCCGAGGGG TCGCCCTCCA GGAAGCGGTG
61       ATGCTGTTGC TGTACTTCGG GGTACACTTC AGGGTACCAA CCGGGCACTC CTACAACCTG
121      GACCCGGAGA GTGCACTGCT GTACCAGGGC CCCTCCGGCA CGCTGTTTGG CTACTCGGTG
181      GTGCTGCACA GCCACGGGAC GAAGCGCTGG CTCATCGTGG GGGCGCCCAC TGCCAGCTGG
241      CTCGCCAATG CCTCGGTGGT CAATCCTGGG GCGATTTACA GATGCAGGAT CGGAAAGAAT
301      CCAAACCAGA TCTGCGAACA GCTCCAGCTG GGAAGCCCCA GTGGAGAACC CTGTGGGAAG
361      ACGTGCTTGG AAGAGAGAGA TAATCAGTGG CTGGGAGTGA CAATTTCCAG ACAGCCAGGA
421      GAAAATGGAT CTATCGTGAC TTGTGGGCAC AGGTGGAAAA ATATATTTTA CATGAAGAAC
481      AATAATAAAC TCCCCACTGG TGTTTGCTAT GGCATGCCTT CTGATTTGCG GACAGAACTA
541      AGTAAAAGGA TGGCCCCCTG TTACCAAGAT TTTGTGAGAA AATTTGGAGA AAATTTTGCA
601      TCGTGTCAAG CTGGAATATC TAGTTTTTAC ACACAGGATT TGATTGTGAT GGGGGCCCCG
661      GGATCATCGT ACTGGACCGG CTCCGTCTTT GTCTACAACA TAACTACGAA CAAATACAAG
721      GCATTTGTAG ACAGACAGAA TCAAGTAAAA TTTGGAAGCT ACTTAGGCTA CTCAGTAGGA
781      GCTGGTCATT TCCGAAATCC ACATACTACC GAAGTCGTGG GAGGAGCCCC TCAACACGAA
841      CAGATAGGAA AAGCATATAT ATTCAGCATT GATGAAAGCG AACTGACCAT CGTATATGAA
901      ATGAAAGGTA AAAAGCTTGG CTCTTACTTT GGAGCTTCTG TCTGTGCGGT GGACCTCAAT
961      GCAGATGGCT TCTCAGATCT CCTTGTTGGA GCGCCTATGC AGAGCACCAT CAGGGAGGAA
1021     GGAAGAGTGT TCGTGTACAT CAACTCTGGC ATGGGAGCTG TGATGGTTGA AATGGAAAGA
1081     GTGCTCGTCG GAAGTGACAA ATATGCTGCC AGGTTTGGGG AATCCATGGC AAATCTTGGC
1141     GACATTGACA ATGATGGCTT TGAAGATATT GCTATTGGCG CACCACAAGA AGATGACTTG
1201     CGAGGCGCTG TCTACATTTA CAATGGCCGA GTAGATGGCA TCTCCTCTAC ATACTCACAG
1261     AGAATCGAAG GACGTCAAAT CAGCCAGTCA CTAAGGATGT TTGGACAGTC TATCTCAGGA
1321     CAAATTGATG CAGACAACAA TGGATATGTT GATGTAGCGG TTGGTGCATT TCAGTCTGAT
1381     TCTGCAGTGT TGCTAAGGAC ACGGCCTGTA GTGATTGTTG AAGCATCTTT AAGCCATCCT
1441     GAGTCAGTAA ATAGAACAAA ATTTGACTGT ACTGAAAATG GACTTCTGTC TGTGTGCGTG
1501     GATCTTAAAC TGTGTTTCTC ATATAAGGGC AAAGAGGTCC CAGGCTACAT CGTTTTGTTT
1561     TACAATGTGA GCTTGGATGT GCACAGGAAG GCCGAGTCTC CGTCAAGATT TTATTTCTTC
1621     TCCAATGGGA CTTCTGACAT GATTACAAGA AGCATACGAG TTTCAAGCAG TGGAGAGAAA
1681     TGTAGGACAC ACCAGGCATT CATGCGGAAA GACGTGCGAG ACATCCTCAC TCCCATTCAG
1741     GTTGAGGCCA CATACCACCT TGGGCATCAT GTGATCACCA AACGAAACAC AGAGGAATTC
1801     CCACCCCTCC AGCCGATCCT TCAGCAGAAG AAAGAAAAAG ACATTATTAG AAAAACGATA
1861     AACTTCGCAA GGTTTTGTGC CTATGAAAAT TGTTCTGCTG ATCTGCAGGT TTCTGCAAAA
1921     GTTGGGTTTT TGAAGCCATA TGAAAATAAA ACCTATCTTG CTGTTGGGAG CATGAAGACC
1981     ATAATGCTAA ATGTGTCCTT GTTCAATGCT GGAGATGATG CTTATGAAAC TACTCTGAAT
2041     GTCCAACTCC CCACAGGCCT TTATTTCATT AAGATCTTAG ACCTGGAAGA GAAACAAATA
2101     AACTGCGACG TGACAGAGAG TTCAGGCGTA GTTAAGCTTG CCTGCAGCCT CGGTTACATA
2161     TACGTGGATC GTCTCTCAAG GATAGACATT AGCTTTCTCC TGGATGTGAG CTCACTCAGC
2221     AGGGCCCATG AGGACCTCAG CATCAACGTG CATGCCTCCT GTGAAAATGA GGGTAAATTG
2281     GACCAAGTGA AAGACAACAG AGTGACCTTA GTGATACCTC TAAGGTATGA AGTTATGCTG
2341     GCTGTTCACG GGTTTGTGAC CCCAACTTCG TTTGTGTATG GATCCAGTGA AGAAAACGAG
2401     CCAGAAATGT GCATGGCAGA GAAATTGAAC CTCACTTTCC ACGTTATAAA CACTGGCATT
2461     AGCATGGCTC CAAATGTTAG TGTGAAAATA ATGGTACCAA ATTCTTTTCT CCCTCGAAAT
2521     GATAAGTTGT TCAACGTTTT GGATGTCCAG GCAACTATGG GGCAGTGCCA ATTTGAACAC
2581     TATGGACGAG AGTGTGCCTT AGCACAGCAA AAAGGCATAG CGGGGACCTT GACCGATTTG
2641     GTCAAATTTC TATCAAAGAC TGATAAGAGA CTCCTGTATT GCATGAAAGC TGATCGACAC
2701     TGTTTAGATT TCTTATGTAA TTTCGGGAAA ATGGAAAGTG GGAAGGAAGC CAGCGTTCAC
2761     ATTCAGCTGG AAGGCAGGCC ATCCGTCTTG GAAATGGATG AGACCTCATC GCTCAAATTT
2821     GAAATAAAAG CAACAGCTTT TCCAGAGCCA CACCCAAAAG TTATTGAGCT CAATAAGGAT
2881     GAGAACATTG CCCATGTTTA CTTGGAAGGG CTCCATCATC AAAGACCCAA ACGGCATTTC
2941     ACCATCGTGA TTGTCACCAT CAGCTTGCTC CTTGGACTTA TTGTACTTTT ATTAATTTCA
3001     TGTGTTATGT GGAAGGCTGG ATTTTTTAAA AGGCAGTACA AATCTATCCT ACAAGAAGAA
3061     AACAGGAGAG ACAGCTGGAG TTATGTCAAC AGCAAAAGCA ATGATGACTG A

Quality Control:cDNA sequencing results is available upon request.