Human ITGA2 ORF Expression Clone, FLAG-tagged

ITGA2 cDNA Clone Datasheet

ITGA2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_002203.3
• cDNA Size: 3573
• Gene Synonym: BR; GPIa; CD49B; VLA-2; VLAA2; ITGA2
• Source: Human

ITGA2 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) DNA.
• Vector: pCMV/hygro-FLAG
• FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for three point mutations: 759 C/T, 825 G/A and 3252 C/T not causing the amino acid variation.

ITGA2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ITGA2 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ITGA2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGCCAG AACGGACAGG GGCCGCGCCG CTGCCGCTGC TGCTGGTGTT AGCGCTCAGT
61       CAAGGCATTT TAAATTGTTG TTTGGCCTAC AATGTTGGTC TCCCAGAAGC AAAAATATTT
121      TCCGGTCCTT CAAGTGAACA GTTTGGCTAT GCAGTGCAGC AGTTTATAAA TCCAAAAGGC
181      AACTGGTTAC TGGTTGGTTC ACCCTGGAGT GGCTTTCCTG AGAACCGAAT GGGAGATGTG
241      TATAAATGTC CTGTTGACCT ATCCACTGCC ACATGTGAAA AACTAAATTT GCAAACTTCA
301      ACAAGCATTC CAAATGTTAC TGAGATGAAA ACCAACATGA GCCTCGGCTT GATCCTCACC
361      AGGAACATGG GAACTGGAGG TTTTCTCACA TGTGGTCCTC TGTGGGCACA GCAATGTGGG
421      AATCAGTATT ACACAACGGG TGTGTGTTCT GACATCAGTC CTGATTTTCA GCTCTCAGCC
481      AGCTTCTCAC CTGCAACTCA GCCCTGCCCT TCCCTCATAG ATGTTGTGGT TGTGTGTGAT
541      GAATCAAATA GTATTTATCC TTGGGATGCA GTAAAGAATT TTTTGGAAAA ATTTGTACAA
601      GGCCTGGATA TAGGCCCCAC AAAGACACAG GTGGGGTTAA TTCAGTATGC CAATAATCCA
661      AGAGTTGTGT TTAACTTGAA CACATATAAA ACCAAAGAAG AAATGATTGT AGCAACATCC
721      CAGACATCCC AATATGGTGG GGACCTCACA AACACATTTG GAGCAATTCA ATATGCAAGA
781      AAATATGCTT ATTCAGCAGC TTCTGGTGGG CGACGAAGTG CTACAAAAGT AATGGTAGTT
841      GTAACTGACG GTGAATCACA TGATGGTTCA ATGTTGAAAG CTGTGATTGA TCAATGCAAC
901      CATGACAATA TACTGAGGTT TGGCATAGCA GTTCTTGGGT ACTTAAACAG AAACGCCCTT
961      GATACTAAAA ATTTAATAAA AGAAATAAAA GCAATCGCTA GTATTCCAAC AGAAAGATAC
1021     TTTTTCAATG TGTCTGATGA AGCAGCTCTA CTAGAAAAGG CTGGGACATT AGGAGAACAA
1081     ATTTTCAGCA TTGAAGGTAC TGTTCAAGGA GGAGACAACT TTCAGATGGA AATGTCACAA
1141     GTGGGATTCA GTGCAGATTA CTCTTCTCAA AATGATATTC TGATGCTGGG TGCAGTGGGA
1201     GCTTTTGGCT GGAGTGGGAC CATTGTCCAG AAGACATCTC ATGGCCATTT GATCTTTCCT
1261     AAACAAGCCT TTGACCAAAT TCTGCAGGAC AGAAATCACA GTTCATATTT AGGTTACTCT
1321     GTGGCTGCAA TTTCTACTGG AGAAAGCACT CACTTTGTTG CTGGTGCTCC TCGGGCAAAT
1381     TATACCGGCC AGATAGTGCT ATATAGTGTG AATGAGAATG GCAATATCAC GGTTATTCAG
1441     GCTCACCGAG GTGACCAGAT TGGCTCCTAT TTTGGTAGTG TGCTGTGTTC AGTTGATGTG
1501     GATAAAGACA CCATTACAGA CGTGCTCTTG GTAGGTGCAC CAATGTACAT GAGTGACCTA
1561     AAGAAAGAGG AAGGAAGAGT CTACCTGTTT ACTATCAAAG AGGGCATTTT GGGTCAGCAC
1621     CAATTTCTTG AAGGCCCCGA GGGCATTGAA AACACTCGAT TTGGTTCAGC AATTGCAGCT
1681     CTTTCAGACA TCAACATGGA TGGCTTTAAT GATGTGATTG TTGGTTCACC ACTAGAAAAT
1741     CAGAATTCTG GAGCTGTATA CATTTACAAT GGTCATCAGG GCACTATCCG CACAAAGTAT
1801     TCCCAGAAAA TCTTGGGATC CGATGGAGCC TTTAGGAGCC ATCTCCAGTA CTTTGGGAGG
1861     TCCTTGGATG GCTATGGAGA TTTAAATGGG GATTCCATCA CCGATGTGTC TATTGGTGCC
1921     TTTGGACAAG TGGTTCAACT CTGGTCACAA AGTATTGCTG ATGTAGCTAT AGAAGCTTCA
1981     TTCACACCAG AAAAAATCAC TTTGGTCAAC AAGAATGCTC AGATAATTCT CAAACTCTGC
2041     TTCAGTGCAA AGTTCAGACC TACTAAGCAA AACAATCAAG TGGCCATTGT ATATAACATC
2101     ACACTTGATG CAGATGGATT TTCATCCAGA GTAACCTCCA GGGGGTTATT TAAAGAAAAC
2161     AATGAAAGGT GCCTGCAGAA GAATATGGTA GTAAATCAAG CACAGAGTTG CCCCGAGCAC
2221     ATCATTTATA TACAGGAGCC CTCTGATGTT GTCAACTCTT TGGATTTGCG TGTGGACATC
2281     AGTCTGGAAA ACCCTGGCAC TAGCCCTGCC CTTGAAGCCT ATTCTGAGAC TGCCAAGGTC
2341     TTCAGTATTC CTTTCCACAA AGACTGTGGT GAGGACGGAC TTTGCATTTC TGATCTAGTC
2401     CTAGATGTCC GACAAATACC AGCTGCTCAA GAACAACCCT TTATTGTCAG CAACCAAAAC
2461     AAAAGGTTAA CATTTTCAGT AACGCTGAAA AATAAAAGGG AAAGTGCATA CAACACTGGA
2521     ATTGTTGTTG ATTTTTCAGA AAACTTGTTT TTTGCATCAT TCTCCCTGCC GGTTGATGGG
2581     ACAGAAGTAA CATGCCAGGT GGCTGCATCT CAGAAGTCTG TTGCCTGCGA TGTAGGCTAC
2641     CCTGCTTTAA AGAGAGAACA ACAGGTGACT TTTACTATTA ACTTTGACTT CAATCTTCAA
2701     AACCTTCAGA ATCAGGCGTC TCTCAGTTTC CAAGCCTTAA GTGAAAGCCA AGAAGAAAAC
2761     AAGGCTGATA ATTTGGTCAA CCTCAAAATT CCTCTCCTGT ATGATGCTGA AATTCACTTA
2821     ACAAGATCTA CCAACATAAA TTTTTATGAA ATCTCTTCGG ATGGGAATGT TCCTTCAATC
2881     GTGCACAGTT TTGAAGATGT TGGTCCAAAA TTCATCTTCT CCCTGAAGGT AACAACAGGA
2941     AGTGTTCCAG TAAGCATGGC AACTGTAATC ATCCACATCC CTCAGTATAC CAAAGAAAAG
3001     AACCCACTGA TGTACCTAAC TGGGGTGCAA ACAGACAAGG CTGGTGACAT CAGTTGTAAT
3061     GCAGATATCA ATCCACTGAA AATAGGACAA ACATCTTCTT CTGTATCTTT CAAAAGTGAA
3121     AATTTCAGGC ACACCAAAGA ATTGAACTGC AGAACTGCTT CCTGTAGTAA TGTTACCTGC
3181     TGGTTGAAAG ACGTTCACAT GAAAGGAGAA TACTTTGTTA ATGTGACTAC CAGAATTTGG
3241     AACGGGACTT TTGCATCATC AACGTTCCAG ACAGTACAGC TAACGGCAGC TGCAGAAATC
3301     AACACCTATA ACCCTGAGAT ATATGTGATT GAAGATAACA CTGTTACGAT TCCCCTGATG
3361     ATAATGAAAC CTGATGAGAA AGCCGAAGTA CCAACAGGAG TTATAATAGG AAGTATAATT
3421     GCTGGAATCC TTTTGCTGTT AGCTCTGGTT GCAATTTTAT GGAAGCTCGG CTTCTTCAAA
3481     AGAAAATATG AAAAGATGAC CAAAAATCCA GATGAGATTG ATGAGACCAC AGAGCTCAGT
3541     AGCGCT1GATT ACAAGGATGA CGACGATAAG2 TAA

 

1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. FLAG Tag

 

 

Quality Control:cDNA sequencing results is available upon request.