Cynomolgus monkey ITGA1 Gene cDNA Clone / ORF Clone

ITGA1 cDNA Clone Datasheet

ITGA1 cDNA Clone Product Information

• cDNA Size: 3540
• Gene Synonym: ITGA1
• Source: Cynomolgus monkey

ITGA1 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) integrin, alpha 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1477 A/G, 1507 T/G resulting in the amino acid Ile substitution by Val, Ser substitution by Ala and 2448 G/A，2466 C/G，2700 C/T not causing the amino acid variation.
  Please check the sequence information before order.

ITGA1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ITGA1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ITGA1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCCCTC GGCCCCGCGC CCGCCCAGGG GTCGCTGTCG CCTGCTGCTG GCTCCTCACT
61       GTTGTTCTAC GCTGCTGCGT ATCATTCAAT GTTGATGTGA AAAATTCGAT GACTTTCAGC
121      GGCCCAGTGG AAGACATGTT TGGATATACT GTTCAACAAT ATGAAAATGA AGAAGGAAAA
181      TGGGTGCTTA TTGGTTCTCC TTTAGTTGGC CAACCCAAAA ACAGAACTGG AGATGTCTAT
241      AAGTGTCCAG TTGGGAGAGG TGAATCATTA CCTTGTGTAA AGATGGATCT ACCAGTTAAT
301      ACATCAATTC CCAATGTCAC AGAAGTAAAA GAGAACATGA CATTTGGATC AACTTTAGTC
361      ACCAACCCAA ACGGAGGATT TTTGGCATGT GGGCCCTTAT ATGCCTATAG ATGTGGACAT
421      TTGCATTACA CAACTGGAAT CTGTTCTGAC GTCAGTCCCA CATTTCAAGT CGTGAATTCC
481      ATTGCCCCTG TAAGAGAATG CAGCACTCAA CTGGACATAG TCATAGTGCT AGATGGTTCC
541      AACAGTATTT ACCCATGGGA CAGTGTTACA GCTTTTTTAA ATGACCTTCT TGAAAGAATG
601      GATATTGGTC CTAAACAGAC ACAGGTTGGA ATTGTACAGT ATGGAGAAAA CGTGACCCAT
661      GAGTTCAACC TCAATAAGTA TTCTTCCACC GAAGAGGTAC TTGTTGCAGC AAAGAAAATA
721      GTCCAGAGAG GTGGCCGCCA GACTATGACA GCTCTTGGAA TAGACACAGC AAGAAAGGAG
781      GCATTCACGG AAGCCCGGGG TGCCCGAAGA GGAGTTAAAA AAGTCATGGT TATTGTGACG
841      GATGGAGAGT CCCATGACAA CCATCGACTG AAGAAGGTCA TTCAAGACTG TGAAGATGAA
901      AACATTCAAC GGTTTTCCAT AGCTATTCTT GGCAGCTATA ACCGAGGAAA TTTAAGCACT
961      GAAAAATTTG TGGAGGAAAT AAAATCAATT GCAAGCGAAC CCACTGAAAA GCATTTCTTC
1021     AATGTCTCTG ATGAATTGGC TCTAGTCACC ATTGTTAAAA CTCTGGGAGA AAGAATATTT
1081     GCCCTGGAAG CTACAGCTGA CCAGTCAGCA GCTTCATTTG AAATGGAAAT GTCTCAGACT
1141     GGCTTCAGTG CTCATTATTC ACAGGACTGG GTCATGCTTG GAGCAGTAGG AGCCTATGAT
1201     TGGAATGGAA CAGTTGTCAT GCAGAAGGCT AGTCAAATAA TCATCCCTCG AAACACAACC
1261     TTTAACGTTG AGTCTACCAA AAAGAATGAA CCGCTTGCTT CTTATTTAGG TTACACTGTA
1321     AACTCTGCTA CTGCTTCTTC TGGAGATGTG CTCTATATTG CTGGACAGCC TCGGTACAAT
1381     CATACAGGCC AGGTCATTAT CTACAGGATG GAAGATGGAA ACATCAAAAT TCTCCAGACA
1441     CTCAGTGGAG AACAGATTGG TTCCTACTTT GGCAGTGTTT TAACAACAAT TGACATTGAC
1501     AAGGATTCTA ATACTGACAT TCTTCTAGTC GGAGCCCCTA TGTACATGGG AACGGAGAAA
1561     GAGGAGCAAG GAAAAGTGTA TGTGTATGCT CTCAATCAGA CAAGGTTTGA ATATCAAATG
1621     AGCCTGGAAC CTACTAAGCA GACGTGCTGT TCATCTCGGC AGCACAATTC ATGCACAAAA
1681     GAAAACAAAA ATGAGCCATG CGGGGCTCGT TTTGGAACTG CTATTGCTGC TGTAAAAGAT
1741     CTCAATCTTG ATGGATTTAA TGACATCGTG ATAGGAGCTC CGCTGGAAGA TGATCACGGG
1801     GGAGCTGTGT ACATTTATCA TGGAAGTGGC AAGACGATAA GGAAAGAGTA TGCACAACGT
1861     ATTCCATCAG GTGGGGATGG TGAGACACTG AAATTTTTTG GCCAGTCTAT CCACGGAGAA
1921     ATGGATTTAA ATGGTGACGG CCTGACAGAT GTGACTATTG GGGGCCTTGG TGGTGCTGCC
1981     CTCTTCTGGT CCCGAGATGT GGCCGTAGTT AAAGTGACCA TGAATTTTGA ACCAAATAAA
2041     GTGAATATTC AAAAGAAAAA CTGCCATATG GAGGGAAAGG AAACAGTATG CATAAATGCT
2101     ACAGTGTGTT TTGATGTGAA ATTAAAATCT AAAGAAGACA CGATTTATGA AGCTGATTTG
2161     CAGTACCGTG TCACCCTAGA TTCACTAAGG CAAATATCAC GAAGTTTTTT CTCTGGAACT
2221     CAAGAGAGAA AAGTTCAAAG GAACATCACA GTTCGAAAAT CAGAATGCAC TAAGCACTCC
2281     TTCTACATGT TGGACAAGCA TGACTTTCAG GACTCTGTGA GAATAACGTT GGACTTTAAT
2341     CTTACCGATC CAGAAAATGG GCCTGTTCTT GATGATTCTC TACCGAACTC AGTACATGAA
2401     TATATTCCTT TTGCCAAAGA TTGTGGAAAC AAGGAAAAAT GTATCTCAGA CCTCAGCCTG
2461     CATGTGGCCA CCACAGAAAA GGACCTGCTA ATTGTCCGGT CCCAGAATGA TAAGTTCAAT
2521     GTTAGCCTCA CAGTCAAAAA TACAAAGGAC AGTGCCTACA ATACCAGGAC AATAGTGCAT
2581     TATTCTCCAA ATCTAGTTTT TTCAGGAATT GAGGCTATCC AAAAAGACAG TTGTGAATCC
2641     AATCATAATA TCACATGTAA AGTTGGATAT CCCTTCCTGA GAAAAGGAGA GATGGTAACT
2701     TTCAAAATAT TGTTTCAGTT TAACACATCC TATCTCATGG AAAATGTGAC CATTTATTTA
2761     AATGCAACAA GCGACAGTGA AGAACCTCCT GAAACCCTTT CTGATAATGT AGTAAACATT
2821     TCTATTCCGG TAAAATATGA AGTTGGACTA CAGTTTTACA GCTCTGCAAG TGAATACCAC
2881     ATTTCAATTG CTGCCAATGA GACAGTCCCT GAAGTTATTA ATTCTACGGA GGACATTGGA
2941     AATGAAATTA ATATCTTCTA CCTGATTAGA AAAAGTGGAT CTTTTCCAAT GCCAGAACTT
3001     AAGCTGTCAA TTTCATTCCC CAACATGACA TCAAATGGTT ATCCTGTGCT ATACCCAACT
3061     GGATTGTCAT CTTCTGATAA TGCAAACTGC AGACCCCATA TCTTTGAGGA TCCTTTCAGT
3121     ATAGACTCTG GAAAGAAAAT GACTACATCA ACTGACCATC TCAAACAAGG CACAATTCTG
3181     GACTGCAATC CATGTAAATT TGCTACCATC ACATGTAATC TCACTCCTTC TGACATGAGC
3241     CAAGTGAATG TTTCACTTAT CTTGTGGAAA CCAACTTTTA TAAAATCATA TTTTTCCAGC
3301     TTAAATCTTA CTATAAGGGG AGAACTTCGG AGTGAAAATA CATCACTGGT TTTAAGTAGC
3361     AGCAATCAAA AAAGAGAGCT TGCTATTCAA ATATCCAAAG ATGGACTACC GGGCAGAGTG
3421     CCATTATGGG TCATCCTGCT GAGTGCTTTT GCTGGATTAT TGCTGTTAAT GCTGCTCATT
3481     TTAGCACTGT GGAAGATTGG ATTCTTCAAA AGACCACTGA AAAAGAAAAT GGAGAAATGA

 

Quality Control:cDNA sequencing results is available upon request.