- EGFR Signaling Pathway
- TGF-beta Signaling
- Canonical Wnt Signaling
- non-Canonical Wnt Signaling
- Notch Signaling
- p53 Pathway
- NF-kB Pathway
- Cytokine Signaling
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ITCH / AIP4 Antibody Datasheet
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ITCH / AIP4 Antibody Product Information
Recombinant Human ITCH / AIP4 protein (Catalog#11131-HNCE)
|Reagents :||FITC-conjugated mouse monoclonal antibody|
Clone ID :
|Ig Type :||
10 μl/Test, 0.1 mg/ml
|Formulation :||Aqueous solution containing 0.5% BSA and 0.09% sodium azide|
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ITCH / AIP4 (rh ITCH / AIP4; Catalog#11131-HNCE; NP_113671.3; Arg 526-Glu 903) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
ITCH / AIP4 Antibody Usage Guide
Human ITCH / AIP4
|Flow Cytometry :||
Flow cytometric analysis of Human ITCH expression in HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and then stained with FITC Mouse anti-ITCH (11131-MM01-F). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.Flow cytometry was performed on a BD FACSCalibur flow cytometry system
Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
|Storage :||This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
ITCH / AIP4 Antibody Related Products & Topics
ITCH / AIP4 Antibody Related Areas:
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|ITCH/AIP4||Human||ITCH/AIP4 (aa 526 - 903) Protein, Recombinant||11131-HNCE|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|Human ITCH/AIP4||WB, ELISA||ITCH / AIP4 Antibody||11131-RP01|
|Human ITCH/AIP4||WB, ELISA||ITCH / AIP4 Antibody (Antigen Affinity Purified)||11131-RP02|
|Human ITCH/AIP4||FCM||ITCH / AIP4 Antibody ( FITC )||11131-MM01-F|
ITCH / AIP4 Antibody Background
- Marchese A. et al., 2003, Dev. Cell 5:709-22.
- Wang Y. et al., 2006, EMBO J. 25: 5058-70.
- Bhandari D. et al., 2009, Mol. Biol. Cell 20: 1324-39.
- Edwards TL. et al., 2009, Biochem. J. 423: 31-9.
- Zhang P. et al., 2010, J. Biol. Chem. 285: 8869-79.
- Azakir B.A. et al., 2010, FEBS J. 277: 1319-30.