Anti-ITCH Antibody (FITC)

Cat: 11131-MM01-F
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All ITCH Reagents
CRO Services
Product recommended

All ITCH Reagents
Anti-ITCH Antibody (FITC) (Mouse Monoclonal antibody) General Information
Product name
Anti-ITCH Antibody (FITC)
Validated applications
FCM
Species reactivity
Reacts with: Human
Specificity
Human ITCH
Immunogen
Recombinant Human ITCH / AIP4 protein (Catalog#11131-HNCE)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ITCH / AIP4 (rh ITCH / AIP4; Catalog#11131-HNCE; NP_113671.3; Arg 526-Glu 903) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Source
Monoclonal Mouse IgG1 Clone #01
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Conjugate
FITC
Concentration
10 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Anti-ITCH Antibody (FITC) (Mouse Monoclonal antibody) Validated Applications

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-ITCH Antibody (FITC) (Mouse Monoclonal antibody) Images
Human ITCH/AIP4 Flow Cytometry (FC) 4842
Flow cytometric analysis of Human ITCH expression in HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and then stained with FITC Mouse anti-ITCH (11131-MM01-F). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Anti-ITCH Antibody (FITC) Alternative Names
Anti-ADMFD Antibody;Anti-AIF4 Antibody;Anti-AIP4 Antibody;Anti-dJ468O1.1 Antibody;Anti-NAPP1 Antibody
ITCH Background Information

E3 ubiquitin-protein ligase Itchy homolog, also known as Atrophin-1-interacting protein 4, NFE2-associated polypeptide 1, NAPP1 and ITCH, is a cell membrane protein which contains one C2 domain, one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and contains four WW domains. ITCH acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. ITCH is involved in the control of inflammatory signaling pathways. It is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. ITCH promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteosomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Defects in ITCH are the cause of syndromic multisystem autoimmune disease (SMAD) which is characterized by organomegaly, failure to thrive, developmental delay, dysmorphic features and autoimmune inflammatory cell infiltration of the lungs, liver and gut.

Full Name
itchy E3 ubiquitin protein ligase
References
  • Marchese A. et al., 2003, Dev. Cell 5:709-22.
  • Wang Y. et al., 2006, EMBO J. 25: 5058-70.
  • Bhandari D. et al., 2009, Mol. Biol. Cell 20:1324-39.
  • Edwards TL. et al., 2009, Biochem. J. 423:31-9.
  • Zhang P. et al., 2010, J. Biol. Chem. 285:8869-79.
  • Azakir B.A. et al., 2010, FEBS J. 277:1319-30.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-ITCH Antibody Mouse Monoclonal FCM,ICC/IF,IF 11131-MM01
    Anti-ITCH Antibody Rabbit Polyclonal ELISA 11131-RP02
    Anti-ITCH Antibody (PE) Mouse Monoclonal FCM 11131-MM01-P
    Anti-ITCH Antibody Rabbit Polyclonal ELISA 11131-RP01
    info info
    添加购物车成功! 添加购物车失败!