Anti-ITCH Antibody (FITC) (Mouse Monoclonal antibody) General Information
Anti-ITCH Antibody (FITC)
Reacts with: Human
Recombinant Human ITCH / AIP4 protein (Catalog#11131-HNCE)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ITCH / AIP4 (rh ITCH / AIP4; Catalog#11131-HNCE; NP_113671.3; Arg 526-Glu 903) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Monoclonal Mouse IgG1 Clone #01
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
10 μl/Test, 0.1 mg/ml
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Anti-ITCH Antibody (FITC) (Mouse Monoclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-ITCH Antibody (FITC) (Mouse Monoclonal antibody) Images
Flow cytometric analysis of Human ITCH expression in HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and then stained with FITC Mouse anti-ITCH (11131-MM01-F). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Anti-ITCH Antibody (FITC) Alternative Names
Anti-ADMFD Antibody;Anti-AIF4 Antibody;Anti-AIP4 Antibody;Anti-dJ468O1.1 Antibody;Anti-NAPP1 Antibody
ITCH Background Information
E3 ubiquitin-protein ligase Itchy homolog, also known as Atrophin-1-interacting protein 4, NFE2-associated polypeptide 1, NAPP1 and ITCH, is a cell membrane protein which contains one C2 domain, one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and contains four WW domains. ITCH acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. ITCH is involved in the control of inflammatory signaling pathways. It is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. ITCH promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteosomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Defects in ITCH are the cause of syndromic multisystem autoimmune disease (SMAD) which is characterized by organomegaly, failure to thrive, developmental delay, dysmorphic features and autoimmune inflammatory cell infiltration of the lungs, liver and gut.
itchy E3 ubiquitin protein ligase
Marchese A. et al., 2003, Dev. Cell 5:709-22. Wang Y. et al., 2006, EMBO J. 25: 5058-70. Bhandari D. et al., 2009, Mol. Biol. Cell 20:1324-39. Edwards TL. et al., 2009, Biochem. J. 423:31-9. Zhang P. et al., 2010, J. Biol. Chem. 285:8869-79. Azakir B.A. et al., 2010, FEBS J. 277:1319-30.