Human ISL1 Gene cDNA Clone / ORF Clone

Human ISL1 cDNA Clone Datasheet

ISL1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_002202.2
• cDNA Size: 1050
• Gene Synonym: Isl-1; ISLET1; ISL1
• Source: Human

ISL1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens ISL LIM homeobox 1 DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

ISL1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ISL1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ISL1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGAGACA TGGGAGATCC ACCAAAAAAA AAACGTCTGA TTTCCCTATG TGTTGGTTGC
61       GGCAATCAGA TTCACGATCA GTATATTCTG AGGGTTTCTC CGGATTTGGA ATGGCATGCG
121      GCATGTTTGA AATGTGCGGA GTGTAATCAG TATTTGGACG AGAGCTGTAC ATGCTTTGTT
181      AGGGATGGGA AAACCTACTG TAAAAGAGAT TATATCAGGT TGTACGGGAT CAAATGCGCC
241      AAGTGCAGCA TCGGCTTCAG CAAGAACGAC TTCGTGATGC GTGCCCGCTC CAAGGTGTAT
301      CACATCGAGT GTTTCCGCTG TGTGGCCTGC AGCCGCCAGC TCATCCCTGG GGACGAATTT
361      GCGCTTCGGG AGGACGGTCT CTTCTGCCGA GCAGACCACG ATGTGGTGGA GAGGGCCAGT
421      CTAGGCGCTG GCGACCCGCT CAGTCCCCTG CATCCAGCGC GGCCACTGCA AATGGCAGCG
481      GAGCCCATCT CCGCCAGGCA GCCAGCCCTG CGGCCCCACG TCCACAAGCA GCCGGAGAAG
541      ACCACCCGCG TGCGGACTGT GCTGAACGAG AAGCAGCTGC ACACCTTGCG GACCTGCTAC
601      GCCGCAAACC CGCGGCCAGA TGCGCTCATG AAGGAGCAAC TGGTAGAGAT GACGGGCCTC
661      AGTCCCCGTG TGATCCGGGT CTGGTTTCAA AACAAGCGGT GCAAGGACAA GAAGCGAAGC
721      ATCATGATGA AGCAACTCCA GCAGCAGCAG CCCAATGACA AAACTAATAT CCAGGGGATG
781      ACAGGAACTC CCATGGTGGC TGCCAGTCCA GAGAGACACG ACGGTGGCTT ACAGGCTAAC
841      CCAGTGGAAG TACAAAGTTA CCAGCCACCT TGGAAAGTAC TGAGCGACTT CGCCTTGCAG
901      AGTGACATAG ATCAGCCTGC TTTTCAGCAA CTGGTCAATT TTTCAGAAGG AGGACCGGGC
961      TCTAATTCCA CTGGCAGTGA AGTAGCATCA ATGTCCTCTC AACTTCCAGA TACACCTAAC
1021     AGCATGGTAG CCAGTCCTAT TGAGGCATGA

 

Quality Control:cDNA sequencing results is available upon request.