ISG15 Antibody (Antigen Affinity Purified)

ISG15 Antibody ( Antigen Affinity Purified ) Datasheet

ISG15 Antibody Product Information

• Immunogen: Recombinant Human ISG15 protein (Catalog#12729-HNAE)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human ISG15 (rh ISG15; Catalog#12729-HNAE; AAH09507.1; Met 1-Ser 165). ISG15 specific IgG was purified by Human ISG15 affinity chromatography.

ISG15 Antibody Usage Guide

• Specificity: Human ISG15
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect pro form of Human ISG15 in WB. Using a DAB detection system, the detection limit for pro form of Human ISG15 is approximately 1 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect pro form of Human ISG15. The detection limit for pro form of Human ISG15 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

ISG15 Antibody Background

Ubiquitin-like protein ISG15, also known as Interferon-induced 15 kDa protein, Interferon-induced 17 kDa protein, Ubiquitin cross-reactive protein, ISG15 and G1P2, is a cytoplasm and secreted protein which is detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons. ISG15 / G1P2 contains 2 ubiquitin-like domains. ISG15 / G1P2 is a ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. ISG15 / G1P2 enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 / G1P2 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. ISG15 / G1P2 Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. ISG15 / G1P2 is deconjugated by USP18/UBP43. It shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. ISG15 / G1P2 may serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. ISG15 / G1P2 may also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. ISG15 / G1P2 seems to display antiviral activity during viral infections.

References

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