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ISG15 Antibody ( Antigen Affinity Purified ) Datasheet
|Order or Inquire for ISG15 Antibody product||Quality antibodies||Antibody production services|
|Detection limit is 1 ng/lane in WB|
|Detection limit is 0.039 ng/well in ELISA|
ISG15 Antibody Product Information
Recombinant Human ISG15 protein (Catalog#12729-HNAE)
|Antibody Type :||Rabbit Polyclonal Antibody ( Antibody Purification Platform )|
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS with 5% trehalose|
Produced in rabbits immunized with purified, recombinant Human ISG15 (rh ISG15; Catalog#12729-HNAE; AAH09507.1; Met 1-Ser 165). ISG15 specific IgG was purified by Human ISG15 affinity chromatography.
ISG15 Antibody Usage Guide
|Western blot :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect pro form of Human ISG15 in WB. Using a DAB detection system, the detection limit for pro form of Human ISG15 is approximately 1 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.|
|Direct ELISA :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect pro form of Human ISG15. The detection limit for pro form of Human ISG15 is approximately 0.039 ng/well.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
ISG15 Antibody Related Products & Topics
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|ISG15||Human||G1P2 / ISG15 (mature form) Protein, Recombinant, Native||12729-HNAE1|
|ISG15||Human||ISG15 / G1P2 Protein, Recombinant, Native||12729-HNAE|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|Human ISG15||WB, ELISA||ISG15 Antibody (Antigen Affinity Purified)||12729-RP02|
ISG15 Antibody Background
Ubiquitin-like protein ISG15, also known as Interferon-induced 15 kDa protein, Interferon-induced 17 kDa protein, Ubiquitin cross-reactive protein, ISG15 and G1P2, is a cytoplasm and secreted protein which is detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons. ISG15 / G1P2 contains 2 ubiquitin-like domains. ISG15 / G1P2 is a ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. ISG15 / G1P2 enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 / G1P2 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. ISG15 / G1P2 Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. ISG15 / G1P2 is deconjugated by USP18/UBP43. It shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. ISG15 / G1P2 may serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. ISG15 / G1P2 may also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. ISG15 / G1P2 seems to display antiviral activity during viral infections.
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