Anti-ISG15 Antibody

Cat: 12729-R239
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Anti-ISG15 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-ISG15 Antibody
Validated applications
WB,ELISA,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human ISG15
Immunogen
Recombinant Human ISG15 protein (Catalog#12729-HNAE)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human ISG15 (rh ISG15; Catalog#12729-HNAE; AAH09507.1; Met1-Ser165).
Source
Monoclonal Rabbit IgG Clone #239
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-ISG15 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:2000-1:10000  
ELISA 1:5000-1:10000  
ICC-IF 1:20-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-ISG15 Antibody (Rabbit Monoclonal antibody) Images
Human ISG15 Immunofluorescence(IF) 2868
Immunofluorescence staining of Human ProISG15 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ProISG15 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.
Human ISG15 Western blot (WB) 13514

Anti-ISG15 rabbit monoclonal antibody at 1:5000 dilution

Lane A: Hela Whole Cell Lysate

Lane B: 293 Whole Cell Lysate

Lane C: Daudi Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:18 kDa

Observed band size:15 kDa

Anti-ISG15 Antibody Alternative Names
Anti-G1P2 Antibody;Anti-hUCRP Antibody;Anti-IFI15 Antibody;Anti-IMD38 Antibody;Anti-IP17 Antibody;Anti-UCRP Antibody
ISG15 Background Information

Interferon-induced 17 kDa protein (ISG15), a 15-kDa protein of unique primary amino acid sequence, functions intracellularly as an ubiquitin homologue and a cytokine that induces production of IFN-gamma and augments NK / lymphokine-activated killer cell proliferation and function. ISG15 is secreted from monocytes and lymphocytes. ISG15 is an ubiquitin-like molecule that is strongly upregulated by type I interferons as a primary response to diverse microbial and cellular stress stimuli. Alterations in the ISG15 signalling pathway have also been found in several human tumour entities. In addition to being stimulated by type I interferon, expression of ISG15 is greatly induced by viral or bacterial infection through the Janus kinase / signal transducer and activator of transcription (Jak / STAT) signalling pathway. After induction, ISG15 is secreted by monocytes, B- and T-lymphocytes and fibroblasts. We demonstrate the novel way in which the function of the ISG15 protein is inhibited by influenza B virus, which strongly induces the ISG15 protein: a specific region of the influenza B virus NS1 protein, which includes part of its effector domain, blocks the covalent linkage of ISG15 to its target proteins both in vitro and in infected cells.

Full Name
ISG15 ubiquitin-like modifier
References
  • Cunha J, et al. (1996) In vitro and in vivo secretion of human ISG15, an IFN-induced immunomodulatory cytokine. J Immunol. 157(9): 4100-8.
  • Andersen JB, et al. (2006) The interferon regulated ubiquitin-like protein, ISG15, in tumorigenesis: friend or foe? Cytokine Growth Factor Rev. 17(6): 411-21.
  • Nuran Bektas, et al. (2008) The ubiquitin-like molecule interferon-stimulated gene 15 (ISG15) is a potential prognostic marker in human breast cancer. Breast Cancer Res. 10(4): 58.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-ISG15 Antibody Rabbit Polyclonal ELISA 12729-RP01
    Anti-ISG15 Antibody Rabbit Polyclonal WB,ELISA,IHC-P 12729-RP02
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