Anti-ISG15 Antibody (Rabbit Monoclonal antibody) General Information
Reacts with: Human
Recombinant Human ISG15 protein (Catalog#12729-HNAE)
This antibody was obtained from a rabbit immunized with purified, recombinant Human ISG15 (rh ISG15; Catalog#12729-HNAE; AAH09507.1; Met1-Ser165).
Monoclonal Rabbit IgG Clone #239
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-ISG15 Antibody (Rabbit Monoclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-ISG15 Antibody (Rabbit Monoclonal antibody) Images
Immunofluorescence staining of Human ProISG15 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ProISG15 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.
Anti-ISG15 rabbit monoclonal antibody at 1:5000 dilution
Lane A: Hela Whole Cell Lysate
Lane B: 293 Whole Cell Lysate
Lane C: Daudi Whole Cell Lysate
Lysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Predicted band size:18 kDa
Observed band size:15 kDa
Anti-ISG15 Antibody Alternative Names
Anti-G1P2 Antibody;Anti-hUCRP Antibody;Anti-IFI15 Antibody;Anti-IMD38 Antibody;Anti-IP17 Antibody;Anti-UCRP Antibody
ISG15 Background Information
Interferon-induced 17 kDa protein (ISG15), a 15-kDa protein of unique primary amino acid sequence, functions intracellularly as an ubiquitin homologue and a cytokine that induces production of IFN-gamma and augments NK / lymphokine-activated killer cell proliferation and function. ISG15 is secreted from monocytes and lymphocytes. ISG15 is an ubiquitin-like molecule that is strongly upregulated by type I interferons as a primary response to diverse microbial and cellular stress stimuli. Alterations in the ISG15 signalling pathway have also been found in several human tumour entities. In addition to being stimulated by type I interferon, expression of ISG15 is greatly induced by viral or bacterial infection through the Janus kinase / signal transducer and activator of transcription (Jak / STAT) signalling pathway. After induction, ISG15 is secreted by monocytes, B- and T-lymphocytes and fibroblasts. We demonstrate the novel way in which the function of the ISG15 protein is inhibited by influenza B virus, which strongly induces the ISG15 protein: a specific region of the influenza B virus NS1 protein, which includes part of its effector domain, blocks the covalent linkage of ISG15 to its target proteins both in vitro and in infected cells.
ISG15 ubiquitin-like modifier
Cunha J, et al. (1996) In vitro and in vivo secretion of human ISG15, an IFN-induced immunomodulatory cytokine. J Immunol. 157(9): 4100-8.Andersen JB, et al. (2006) The interferon regulated ubiquitin-like protein, ISG15, in tumorigenesis: friend or foe? Cytokine Growth Factor Rev. 17(6): 411-21.Nuran Bektas, et al. (2008) The ubiquitin-like molecule interferon-stimulated gene 15 (ISG15) is a potential prognostic marker in human breast cancer. Breast Cancer Res. 10(4): 58.