Insulin Receptor / INSR / CD220 Protein Price Inquiry ( Available Sizes )
Insulin Receptor / INSR / CD220 Protein Product Information
||CD220, HHF5, INSR
||A DNA sequence encoding the human INSR isoform long (NP_000199.2) cytoplasmic domain (Gly 989-Ser 1382) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Insulin Receptor / INSR / CD220 Protein QC Testing
||>92 % as determined by SDS-PAGE.
Insulin Receptor / INSR / CD220 protein
||< 1.0 EU per μg of the protein as determined by the LAL method
||Samples are stable for up to twelve months from date of receipt at -70℃
|Predicted N terminal:
||The recombinant human INSR (989-1382)/GST chimera consists of 631 amino acids and has a calculated molecular mass of 72.3 KDa. It migrates as an approximately 70 KDa band in SDS-PAGE under reducing conditions.
||Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.4, 20%gly, 0.3 mM DTT
- Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
- Please contact us for any concerns or special requirements.
Insulin Receptor / INSR / CD220 Protein Usage Guide
||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Insulin Receptor / INSR / CD220 Protein Related Products & Topics
INSR / CD220 Protein Description
INSR (Insulin receptor), also known as CD220, is a transmembrane receptor that is activated by insulin. INSR belongs to theprotein kinase superfamily, and exists as a tetramer consisting of two alpha subunits and two beta subunits linked by disulfide bonds. The alpha and beta subunits are encoded by a single INSR gene, and the beta subunits pass through the cellular membrane. As the receptor for insulin with tyrosine-protein kinase activity, INSR associates with downstream mediators upon binding to insulin, including IRS1 (insulin receptor substrate 1) and phosphatidylinositol 3'-kinase (PI3K). IRS-1 binding and phosphorylation eventually leads to an increase in the high affinity glucose transporter (Glut4) molecules on the outer membrane of insulin-responsive tissues. INSR isoform long and isoform short are expressed in the peripheral nerve, kidney, liver, striated muscle, fibroblasts and skin, and is found as a hybrid receptor with IGF1R which also binds IGF1 in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibrobasts, spleen and placenta. Defects in Insulin Receptor/INSR are the cause of Rabson-Mendenhall syndrome (Mendenhall syndrome), insulin resistance (Ins resistance), leprechaunism (Donohue syndrome), and familial hyperinsulinemic hypoglycemia 5 (HHF5). It may also be associated with noninsulin-dependent diabetes mellitus (NIDDM).
- Kadowaki T. et al., 1990, J Biol Chem. 265:19143-50.
- Kan M. et al., 1995, Diabetes. 44: 1081-6.
- Duckworth WC. et al., 1998, Endocr Rev. 19 (5): 608-24.
- Maddux B A. et al., 2000, Diabetes. 49 (1): 13-9.
- Longo N. et al., 2002, Hum Mol Genet. 11 (12): 1465-75.
- Ward CW. et al., 2009, Bioessays. 31 (4): 422-34.
INSR / CD220 related areas, pathways, and other information