Insulin cDNA ORF Clone, Human, untagged General Information
Gene
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human insulin.
Plasmid
Promoter
Enhanced CMV promoter
Restriction Sites
KpnI + XhoI (5.5kb + 0.33kb)
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Ampicillin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
Insulin cDNA ORF Clone, Human, untagged Alternative Names
IDDM cDNA ORF Clone, Human;IDDM1 cDNA ORF Clone, Human;IDDM2 cDNA ORF Clone, Human;ILPR cDNA ORF Clone, Human;INS cDNA ORF Clone, Human;IRDN cDNA ORF Clone, Human;MODY10 cDNA ORF Clone, Human
Insulin Background Information
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake.
Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
References
Veedfald S, Plamboeck A, Deacon C F, et al. Cephalic phase secretion of insulin and other enteropancreatic hormones in humans[J]. American Journal of Physiology-Gastrointestinal and Liver Physiology, 2016, 310(1): G43-G51.
