Human ILVBL Gene cDNA Clone / ORF Clone

ILVBL cDNA Clone Datasheet

ILVBL cDNA Clone Product Information

• Gene Bank Ref.ID: BC011722
• cDNA Size: 1899
• Gene Synonym: AHAS; 209L8; ILV2H; LVBL
• Source: Human

ILVBL cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens ilvB (bacterial acetolactate synthase)-like DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1899 G/A not causing the amino acid variation.

ILVBL cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ILVBL cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ILVBL cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGACCC CCGCGGCCGC CGCCCCCGCT GGGAGCTTAT TCCCCTCCTT CCTGCTCCTG
61       GCCTGCGGGA CGCTGGTGGC CGCCTTGCTG GGCGCCGCTC ACCGCCTGGG GCTCTTCTAT
121      CAGCTGCTGC ACAAGGTGGA CAAGGCAAGC GTCCGGCATG GCGGAGAGAA CGTGGCCGCT
181      GTGCTGAGGG CCCATGGTGT GCGGTTCATC TTCACGCTGG TCGGTGGGCA CATTTCCCCG
241      CTGCTGGTGG CCTGTGAGAA ACTGGGCATC CGTGTGGTGG ACACACGCCA TGAGGTCACG
301      GCCGTCTTTG CTGCTGATGC TATGGCCCGC CTGTCCGGGA CGGTGGGCGT GGCGGCAGTG
361      ACAGCAGGCC CTGGCCTCAC CAACACGGTG ACTGCGGTGA AGAATGCTCA GATGGCTCAG
421      TCCCCAATCC TGCTTCTGGG TGGGGCTGCC AGCACTCTGC TGCAGAACCG GGGTGCGCTC
481      CAGGCTGTTG ATCAGCTGTC CCTTTTCCGG CCACTCTGTA AGTTTTGTGT GTCTGTGCGG
541      AGGGTGCGGG ACATTGTGCC CACCCTGAGG GCCGCGATGG CTGCCGCCCA GTCGGGCACC
601      CCAGGTCCGG TGTTTGTGGA GCTGCCCGTT GACGTGCTGT ACCCCTACTT CATGGTCCAG
661      AAGGAGATGG TGCCAGCCAA GCCACCCAAG GGCCTCGTGG GCCGAGTGGT CTCCTGGTAT
721      TTAGAGAATT ACCTGGCCAA CCTCTTTGCA GGAGCCTGGG AGCCTCAGCC CGAGGGACCG
781      CTGCCCCTGG ACATCCCCCA GGCTTCCCCG CAGCAGGTTC AGCGCTGTGT GGAGATCCTG
841      AGCCGGGCCA AGAGGCCTCT GATGGTGCTG GGGAGTCAGG CCCTGCTCAC CCCAACGTCT
901      GCCGACAAGC TTCGGGCTGC CGTGGAGACC TTGGGTGTTC CCTGCTTCCT TGGAGGGATG
961      GCACGGGGGC TGTTAGGCCG CAACCACCCC CTCCACATCC GGGAGAACCG CAGTGCGGCC
1021     CTGAAGAAGG CGGACGTCAT TGTCCTAGCA GGAACTGTGT GTGACTTCCG CCTATCCTAT
1081     GGCCGTGTCC TCAGCCACAG CAGCAAGATC ATCATCGTCA ATCGTAATCG GGAAGAGATG
1141     TTGCTCAACT CAGACATCTT CTGGAAGCCC CAGGAGGCTG TGCAGGGAGA TGTGGGTTCC
1201     TTCGTGCTGA AGTTAGTGGA GGGCCTTCAG GGCCAGACCT GGGCCCCAGA CTGGGTGGAG
1261     GAGCTGCGGG AAGCCGACCG GCAGAAGGAG CAGACCTTTC GGGAGAAGGC AGCGATGCCT
1321     GTGGCCCAGC ACCTGAACCC AGTGCAGGTG CTGCAGCTGG TGGAGGAAAC GCTACCTGAC
1381     AACTCAATTC TGGTGGTGGA TGGCGGGGAC TTCGTGGGCA CTGCTGCCCA TCTGGTACAG
1441     CCCCGCGGCC CCCTGCGCTG GCTCGATCCT GGGGCCTTTG GGACTCTGGG AGTTGGTGCA
1501     GGATTTGCAC TTGGGGCCAA GCTGTGCCGG CCAGATGCTG AGGTCTGGTG CCTGTTTGGG
1561     GACGGAGCTT TTGGCTACAG CCTCATCGAA TTTGATACAT TCGTCAGACA CAAGATCCCA
1621     GTGATGGCCT TGGTAGGGAA TGATGCTGGC TGGACACAGA TTTCTCGGGA GCAGGTGCCC
1681     TCTCTGGGCA GCAACGTGGC CTGTGGCCTG GCCTACACTG ATTATCACAA GGCAGCCATG
1741     GGTCTGGGGG CCCGGGGCTT GCTGCTCTCA CGGGAGAACG AGGATCAGGT GGTCAAGGTG
1801     CTGCACGATG CCCAGCAGCA GTGCCGAGAC GGCCACCCGG TTGTGGTCAA CATCCTCATT
1861     GGGAGGACGG ACTTCCGCGA TGGCTCCATT GCTGTATAA

 

Quality Control:cDNA sequencing results is available upon request.