Mouse ILK Gene cDNA Clone / ORF Clone

ILK cDNA Clone Datasheet

ILK cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001161724.1
• cDNA Size: 1359
• Gene Synonym: ESTM24; AA511515; Ilk
• Source: Mouse

ILK cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus integrin linked kinase DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

ILK cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ILK cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ILK cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGACGACA TTTTCACTCA GTGCCGGGAG GGCAACGCGG TGGCGGTGCG CTTGTGGCTG
61       GACAACACAG AGAACGACCT CAATCAGGGG GATGATCATG GCTTCTCCCC CTTGCACTGG
121      GCCTGCCGAG AAGGCCGCTC TGCGGTGGTT GAAATGCTGA TCATGCGTGG AGCACGGATT
181      AATGTGATGA ATCGTGGGGA TGATACCCCC CTGCACCTGG CAGCTAGTCA TGGACACCGT
241      GACATTGTAC AGAAGCTGTT GCAATACAAG GCTGACATCA ATGCAGTGAA TGAGCACGGC
301      AATGTGCCAC TTCACTATGC ATGTTTCTGG GGCCAAGACC AGGTGGCAGA GGACCTGGTG
361      GCTAACGGGG CTCTTGTGAG CATCTGTAAC AAGTATGGAG AGATGCCTGT GGACAAAGCC
421      AAGGCACCCC TTAGAGAGCT TCTCCGAGAA CGGGCAGAGA AAATGGGCCA GAATCTCAAC
481      CGTATTCCAT ACAAGGACAC ATTCTGGAAG GGGACCACTC GCACAAGGCC CCGAAATGGG
541      ACCCTGAACA AACACTCCGG TATTGACTTC AAACAGCTCA ACTTTCTGGC AAAGCTCAAT
601      GAGAATCATT CTGGAGAGCT TTGGAAAGGC CGCTGGCAGG GCAATGATAT TGTTGTGAAG
661      GTGCTGAAGG TTCGAGACTG GAGTACAAGG AAAAGCAGGG ACTTCAATGA GGAATGTCCC
721      CGGCTCAGGA TTTTCTCACA CCCTAACGTG CTCCCAGTGC TAGGTGCTTG CCAGGCTCCC
781      CCAGCCCCCC ACCCAACCCT CATCACACAC TGGATGCCAT ATGGATCTCT CTACAATGTT
841      CTACATGAAG GCACCAATTT CGTTGTGGAC CAGAGCCAAG CTGTAAAGTT TGCTTTGGAC
901      ATGGCAAGAG GCATGGCTTT TCTTCACACA CTAGAGCCTC TCATACCCCG GCATGCACTC
961      AATAGCCGCA GTGTAATGAT CGATGAAGAT ATGACTGCCC GAATCAGCAT GGCTGATGTT
1021     AAGTTTTCTT TCCAGTGCCC TGGGCGCATG TATGCGCCTG CCTGGGTGGC CCCTGAAGCC
1081     CTGCAGAAGA AGCCTGAAGA CACAAACAGA CGCTCAGCAG ACATGTGGAG TTTCGCGGTG
1141     CTTCTGTGGG AACTGGTGAC ACGAGAGGTG CCCTTTGCTG ACCTCTCTAA TATGGAGATT
1201     GGGATGAAGG TGGCACTGGA AGGCCTTCGG CCTACCATCC CACCAGGTAT TTCCCCCCAT
1261     GTGTGTAAGC TCATGAAGAT TTGCATGAAT GAAGACCCTG CAAAGCGACC CAAGTTTGAC
1321     ATGATTGTGC CTATCTTGGA GAAGATGCAG GACAAGTAG

 

Quality Control:cDNA sequencing results is available upon request.