Cynomolgus monkey IL8RB Gene cDNA Clone / ORF Clone (Cytokine)

IL8RB cDNA Clone Datasheet

IL8RB cDNA Clone Product Information

• cDNA Size: 1068
• Gene Synonym: IL8RB
• Source: Cynomolgus monkey

IL8RB cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) c-X-C chemokine receptor type 2-like DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 148 G/A，537 G/C，898 T/C，949 T/C， resulting in the amino acid Val substitution by Ile，Gln substitution by His，Phe substitution by Leu，Cys substitution by Arg and 831 A/C，975 T/C not causing the amino acid variation.
  Please check the sequence information before order.

IL8RB cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL8RB cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL8RB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAAAATT TTAGCTGGGA AAATTACCTG TGGGAAGACA TCTTCAGTGG AGATTTTAGT
61       AATTACAGTT ACAGCCTTGA CCTGCCCCCT TTTCTACTAG ATTCTGCTCC ATGTTGGTCA
121      GAATCCCTAG AAATCAATTA TGTTTTGATC ATCATCTATG CCCTGATGTT TCTACTGAAT
181      CTGATGTGAA ACTCCCTGCT GATGCTGGTT ATCTTATTCA GTTGAGTCAG CCACTCTGTC
241      GCTGATGTCT ACCTGCTGAA CCTGGCCTTG GTCGACCTGT TCTTTTTCCT GACATTGCCC
301      ATCTCAGCTG CCTCCAAGAT GAATGGCTGG ATTTTTGGCA CAATCTGTGC CAGGTGGTCT
361      AGCTCCTGAA GGAAGTCAGC TTCTACAGTG GTATTCTACT ACTGGCCTGC CGCAGCATGG
421      ACTGTTACCT GGCCATTATC CATGCCCCCC ACACACTGAC CCAGCGGCAT CACTTGGTCA
481      AGTTCATATG TCTGGATATG TGGACCCTGT TCCTGTTAGT GGCCCTACCC ATCTCACTTT
541      TCCGAAGGAG CACCCTACGT TAGCCCAGTC TGCTATGAGG ACATGGGCAA CAATACAGCA
601      AACTGGCAGA TGCTGTTACG GATCCTGTCC CAGACCTTTG GCTTCATCGT GCCACTACTG
661      GTCATGCTGT TCTGCTATGG ATTCACCCTG CGCATGCTGT TTAAGGCTCA CATGGGGCAG
721      AAGCACTGGG CCATGCAGGT CATCTTTGCT GTTGTCCTCA TCTTCCTGCT CCGCTGGCTG
781      CCCTACAACC TGGTCCTGCT GGCAGACACC CTCATGAGAA CCCAGATGAT CAAGGAGAGC
841      TGTCAGCGCC GCAACGACAT CGACCAGGCC CTGGATGCCA CCGAGATTCT GGGCATCCTT
901      CACAGCTGCC TCAACCCCCT CATCTACGCC TTCATTGGCC AGAAGTTCCG CCATGGACTC
961      CTCAAGATTC TAGCCACACA TGGCTTGATC AGCAAGGACT CCCTGCCCAA AGACAGCAGG
1021     CCTTCCTTTG TTGGCTCTTC TTCAGGACAC ACTTCCACTA CTCTCTGA

 

Quality Control:cDNA sequencing results is available upon request.