Rat IL8RB Gene cDNA Clone / ORF Clone

IL8RB cDNA Clone Datasheet

IL8RB cDNA Clone Product Information

• Gene Bank Ref.ID: NM_017183.1
• cDNA Size: 1080
• Gene Synonym: Cxcr2, Cmkar2, Il8rb
• Source: Rat

IL8RB cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus interleukin 8 receptor, beta DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 192 G/A and 243 T/C not causing the amino acid variation.

IL8RB cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL8RB cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL8RB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGAGAAA TCAGGGTGGA TAATTTCAGC CTTGAAGACT TCTTCAGTGG AGATATTGAC
61       AGTTACAATT ACAGTTCTGA CCCGCCCTTT ACTCTGTCAG ATGCTGCCCC ATGCCCCTCA
121      GCGAACCTAG ATATCAACAG GTATGCTGTG GTTGTCATAT ACGTTCTGGT GACTTTGCTG
181      AGTCTCGTGG GAAACTCCCT GGTGATGCTG GTCATCTTGT ACAATCGAAG CACCTGCTCT
241      GTCACCGACG TCTACCTGCT GAACCTGGCC ATTGCTGATC TGTTCTTTGC CCTGACCTTG
301      CCTGTCTGGG CTGCATCTAA AGTAAATGGA TGGATTTTTG GCTCATTCCT GTGCAAGGTA
361      TTCTCGTTCC TGCAGGAGAT TACCTTCTAC AGCAGTGTTC TGTTGCTAGC CTGCATCAGC
421      ATGGACCGCT ACCTGGCCAT CGTCCACGCC ACAAGTACAC TGATCCAGAA GAGACACTTG
481      GTCAAGTTTG TGTGCATCAC CATGTGGTTT CTCTCACTAG TTCTGTCCCT GCCCATCTTC
541      ATTCTTCGGA CTACTGTTAA GGCAAACCCT TCTACCGTAG TCTGCTATGA GAATATAGGT
601      AATAATACAT CCAAGTGGAG GGTGGTACTG CGCATCCTGC CTCAGACCTA TGGCTTCCTC
661      CTGCCGCTGC TCATCATGCT GTTCTGCTAT GGGTTCACAC TGCGCACGCT CTTTAAGGCC
721      CACATGGGGC AGAAGCACCG GGCCATGCGG GTCATCTTTG CTGTGGTCCT CGTCTTCCTG
781      CTCTGCTGGC TTCCCTACAA CATTGTCCTG TTCACAGACA CCCTCATGAG AACCAAGCTG
841      ATCAAGGAGA CCTGTGAACG CCAGAACGAG ATTAACAAAG CTTTGGAAGC TACTGAGATT
901      CTTGGCTTCC TCCACAGCTG TCTTAACCCC ATCATCTATG CCTTTATTGG CCAGAAATTT
961      CGCCATGGAC TTCTCAAGAT CATGGCTAAT TATGGCCTTG TCAGCAAGGA GTTCTTAGCC
1021     AAGGAGGGCA GGCCTTCTTT TGTTGGCTCT TCTTCAGCGA ACACCTCCAC TACCCTCTAA

 

Quality Control:cDNA sequencing results is available upon request.