Human EPO Gene cDNA Clone / ORF Clone (Codon Optimized)

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10236-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

EPO cDNA Clone Datasheet

EPO cDNA Clone Product Information

cDNA Size:	582
Gene Synonym:	EPO; EP; MGC138142
Source:	Human

EPO cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens erythropoietin DNA.
Vector:	Cloning Vector: pMD18-T Simple Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with NP_000790.2.

EPO cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EPO cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EPO cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGAGTGC ATGAATGTCC TGCCTGGCTG TGGCTTCTCC TGTCACTGCT GTCTCTCCCT
61       CTGGGCCTCC CAGTGCTGGG CGCACCACCA AGACTCATCT GTGACAGCAG AGTGCTGGAG
121      AGGTATCTCT TGGAGGCCAA GGAGGCTGAG AACATTACCA CAGGCTGTGC TGAACACTGC
181      AGCTTGAATG AGAATATCAC TGTCCCAGAC ACCAAAGTTA ATTTCTATGC CTGGAAGAGG
241      ATGGAGGTTG GGCAACAAGC AGTTGAAGTG TGGCAAGGCC TGGCCCTGCT GTCTGAAGCT
301      GTCCTGAGGG GCCAGGCACT GTTGGTCAAC TCTTCCCAGC CTTGGGAGCC CCTGCAACTG
361      CATGTGGATA AAGCAGTGAG TGGCCTTAGA AGCCTCACCA CTCTGCTTCG GGCTCTGGGA
421      GCACAGAAGG AAGCCATCTC CCCTCCAGAT GCAGCCTCAG CAGCTCCACT CAGAACAATT
481      ACTGCTGACA CTTTTAGAAA ACTCTTTAGG GTGTACTCCA ATTTCCTCCG GGGAAAGCTG
541      AAGCTGTACA CAGGTGAGGC ATGTAGGACA GGGGACAGAT GA

Quality Control:cDNA sequencing results is available upon request.