Rat IL7R / IL7RA Protein (Cytokine)

Interleukin 7 receptor Protein Datasheet

IL7R / IL7RA Protein Product Information

• Synonym: IL7R
• Protein Construction: A DNA sequence encoding the rat IL7R (D4A3X8) (Met1-Asp239) was fused with Fc region of mouse IgG at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

IL7R / IL7RA Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 21
• Molecular Mass: The recombinant rat IL7R /mFc comprises 453 amino acids and has a predicted molecular mass of 51.4 kDa. The apparent molecular mass of the protein is approximately 65 in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL7R / IL7RA protein

IL7R / IL7RA Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL7R / IL7RA Protein Description

Interleukin 7 Receptor alpha ( IL-7Rα ), also known as CD127, is a hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival. IL-7Rα is majorly expressed on T cells and their precursors, and early in B cell development as well, prior to the appearance of surface IgM. Mature human IL-7Rα consists of a 219 aa extracellular domain (ECD) with one fibronectin type-III domain and a WSxWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain. Within the ECD, human IL-7Rα shares 67% aa sequence identity with mouse IL-7Rα. Mutations in the human IL-7Rα gene cause a type of severe combined immunodeficiency in which the major deficiencies are in T cell development, whereas B and NK cells are relatively normal in number. Therefore, the IL-7Rα is critically required for the proper development and function of lymphoid cells. In addition to IL-7, IL-7Rα also associates with TSLPR to form the functional receptor for thymic stromal lymphopoietin (TSLP) which indirectly regulates T cell development by modulating dendritic cell activation. Dynamic regulation of IL-7Rα is important for the generation of appropriate immune responses.

References

1. Sudo T. et al., 1993, Proc. Natl Acad Sci. 90: 9125-9.
2. Park LS. et al., 2000, J Exp Med. 192: 659-70.
3. Pandey A. et al., 2000, Nat Immunol. 1: 59-64.
4. Kaech SM. et al., 2003, Nat Immunol. 4: 1191-8.
5. Gregory SG. et al., 2007, Nat Genet. 39 (9): 1083-91.
6. Mazzucchelli R. et al., 2007, Nat Rev Immunol. 7:144-54.
7. Vranjkovic A. et al., 2007, Int Immunol. 19: 1329-39.