Rat IL7 Gene cDNA Clone / ORF Clone (Cytokine)

IL7 cDNA Clone Datasheet

IL7 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013110.2
• cDNA Size: 465
• Gene Synonym: Il1a; IL-1 alpha
• Source: Rat

IL7 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus interleukin 7 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

IL7 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL7 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL7 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTCCATG TTTCTTTTAG ATATATCTTT GGAATTCCTC CCCTGATCCT TGTGCTGCTG
61       CCGGTCACAT CGTCTGACTG TCACATTAAA GACAAGGACG GTAAAGCCTT TGGGAGTGTG
121      CTGATGATCA GCATCAATCA ACTGGACAAA ATGACAGGAA CTGATAGTGA TTGCCCAAAT
181      AATGAACCAA ACTTTTTTAA AAAACATCTA TGTGATGATA CAAAGGAAGC TGCTTTTCTA
241      AATCGTGCTG CTCGCAAGTT GAGGCAATTT CTTAAAATGA ATATCAGTGA GGAATTCAAT
301      GACCATTTAC TAAGAGTTTC AGACGGCACA CAAACACTGG TGAACTGCAC AAGCAAGGAA
361      GAAAAAACCA TAAAGGAACA GAAAAAGAAT GATCCATGTT TCCTAAAGAG ACTACTGAGA
421      GAGATAAAAA CTTGTTGGAA TAAAATCTTG AACAGCAGTA TATAA

 

Quality Control:cDNA sequencing results is available upon request.