Mouse IL6ST / gp130 / CD130 Protein (Cytokine)

Interleukin 6 signal transducer Protein Datasheet

IL6ST / gp130 / CD130 Protein Product Information

• Synonym: Il6st,5133400A03Rik, AA389424, BB405851, CD130, D13Ertd699e, gp130
• Protein Construction: A DNA sequence encoding the mouse gp130 (NP_034690.3) extracellular domain (Met 1-Glu 617) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

IL6ST / gp130 / CD130 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE.
• Bio-activity: Measured by its ability to inhibit the IL6 Rα enhancement of IL6 activity on M1 mouse myeloid leukemia cells (Saito, T. et al. 1991, J. Immunol. 147:168.).

  The ED50 for this effect is typically 0.03-0.15 μg/ml in the presence of 10 ng/ml recombinant human IL6sR and 20 ng/ml recombinant human IL6.

• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 19
• Molecular Mass: The recombinant mouse gp130/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 843 amino acids and has a predicted molecular mass of 94.7 kDa. As a result of glycosylation, the apparent molecular mass of rm gp130/Fc monomer is approximately 120-130 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL6ST / gp130 / CD130 protein

IL6ST / gp130 / CD130 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL6ST / gp130 / CD130 Protein Description

Mouse glycoprotein 130 (gp130), also known as IL6ST, IL6R-beta and CD130, is a common signal-transducing molecule shared by the functional receptor complexes of the IL-6 family of cytokines (IL-6, IL-11, leukemia inhibitory factor (LIF), granulocyte colonystimulating factor, ciliary neurotrophic factor, and cardiotrophin-1). It belongs to the class â…  cytokine receptor family, and was found in tissues such as brain, heart, thymus, spleen, kidney, lung and liver. Mouse gp130 contains five fibronectin type-III domains, and one Ig-like C2-type (immunoglobulin-like) domain. gp130 is an important part of many different types of signaling complexes. It binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. This complex of 3 proteins then homodimerizes to form a hexameric complex which can produce downstream signals. Inactivation of gp130 is lethal to mice. The mice homozygous for a knock-in mutation in gp130 ( Y757F/Y757F ) that leads to gp130-dependent signal transducers and activators of transcription (STAT) 1/3 hyperactivation develop a broad spectrum of hematopoietic abnormalities, including splenomegaly, lymphadenopathy, and thrombocytosis. gp130 is also involved in various human diseases, such as liver disease, coronary artery disease and myocardial infarction. Furthermore, gp130 is widely expressed in breast cancer cell lines and primary tumors, thus it have been implicated in constitutive STAT3 activation in breast cancer.

References

1. Saito, M. et al., 1992, J Immunol. 148(12): 4066-71.
2. Murakami, M. et al., 1993, Science. 260 (5115): 1808-10.
3. Yoshida, K. et al., 1996, Proc Natl Acad Sci. 93(1): 407-11.
4. Selander, K.S. et al., 2004, Cancer Res. 64(19): 6924-33.
5. Jenkins, B.J. et al., 2005, Blood. 105(9): 3512-20.
6. Luchtefeld, M. et al., 2007, J Exp Med. 204(8): 1935-44.