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Cynomolgus monkey IL6 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog Vector (Price) Quantity In Stock Operation
CG90197-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IL6 cDNA Clone Datasheet

 

IL6 cDNA Clone Product Information

cDNA Size:

639

Gene Synonym:

IL6

Source: Cynomolgus monkey

IL6 cDNA Clone Description

cDNA Description:

ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) interleukin 6 (interferon, beta 2) DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 112 A/G and 614 A/G resulting in the amino acid Asn substitution by Asp, Asn substitution by Ser.
Please check the sequence information before order.

IL6 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL6 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
  3. Transfer the tube to incubation at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL6 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAACTCCT TCTCCACAAG CGCCTTCGGT CCAGTTGCCT TCTCCCTGGG GCTGCTCCTG
61       GTGTTGCCTG CTGCCTTCCC CGCCCCAGTA CTCCCAGGAG AAGATTCCAA AGATGTAGCC
121      GCCCCACACA GCCAGCCACT GACCTCTTCA GAACGAATTG ACAAACACAT TCGGTACATC
181      CTCGACGGCA TCTCAGCCCT GAGAAAGGAG ACATGTAACA GGAGTAACAT GTGTGAAAGC
241      AGCAAAGAGG CACTGGCAGA AAACAACCTG AACCTTCCAA AGATGGCTGA AAAAGATGGA
301      TGCTTCCAAT CTGGATTCAA TGAGGACACT TGCCTGGTGA AAATCATCAC TGGTCTTTTG
361      GAGTTTGAGG TATACCTAGA GTACCTCCAG AACAGGTTTG AGAGTAGTGA GGAGCAAGCC
421      AGAGCTGTGC AAATGAGTAC AAAAGTCCTG ATCCAGTTCC TGCAGAAAAA GGCAAAGAAT
481      CTAGATGCAA TAACCACCCC TGAACCAACC ACAAATGCCA GCCTGCTGAC GAAGCTGCAG
541      GCACAGAACC AGTGGCTGCA GGACATGACG ACGCATCTCA TCCTGCGCAG CTTTAAGGAG
601      TTCCTGCAGT CCAGCCTGAG GGCTCTTCGG CAAATGTAG

 

Quality Control:cDNA sequencing results is available upon request.