|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) interleukin 6 (interferon, beta 2) DNA.|
|Identical with NM_001042756.1 [ Macaca mulatta (Rhesus monkey) ]: 112 A/G and 614 A/G resulting in the amino acid Asn substitution by Asp, Asn substitution by Ser. Please check the sequence information before order.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Cynomolgus monkey IL6 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||CG90197-G-F|
|Cynomolgus monkey IL6 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||CG90197-G-H|
|Cynomolgus monkey IL6 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||CG90197-G-M|
|Cynomolgus monkey IL6 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||CG90197-G-N|
|Cynomolgus monkey IL6 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||CG90197-G-Y|
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Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.