Canine IL6 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
DG70015-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IL6 cDNA Clone Datasheet

IL6 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001003301.1
cDNA Size:	624
Gene Synonym:	IL-6;IL6
Source:	Canine

IL6 cDNA Clone Description

cDNA Description:	ORF Clone of Canis lupus familiaris interleukin 6 (interferon, beta 2) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 540 C/G, 541 G/C resulting in the amino acid Cys substitution by Trp, Val substitution by Leu and 201 T/C not causing the amino acid variation.

IL6 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL6 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL6 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAACTCCC TCTCCACAAG CGCCTTCTCC CTGGGGCTGC TCCTGGTGAT GGCTACTGCT
61       TTCCCTACCC CGGGACCCCT GGCAGGAGAT TCCAAGGATG ATGCCACTTC AAATAGTCTA
121      CCACTCACCT CTGCAAACAA AGTGGAAGAA CTGATTAAGT ACATCCTCGG CAAAATCTCT
181      GCACTGAGAA AGGAGATGTG CGACAAGTTT AACAAGTGTG AAGACAGCAA AGAGGCACTG
241      GCAGAAAATA ACCTACATCT TCCCAAACTG GAGGGAAAAG ATGGATGCTT CCAATCTGGG
301      TTCAATCAGG AGACCTGCTT GACAAGAATC ACTACCGGTC TTGTGGAGTT TCAGCTACAC
361      CTGAATATCC TCCAGAACAA CTATGAGGGT GATAAGGAAA ATGTCAAGTC TGTGCACATG
421      AGTACCAAGA TCCTGGTCCA GATGCTAAAG AGCAAGGTAA AGAATCAGGA TGAAGTGACC
481      ACTCCTGACC CAACCACAGA CGCCAGCCTG CAGGCTATCT TGCAGTCGCA GGATGAGTGG
541      CTGAAGCACA CAACAATTCA CCTCATCCTG CGGAGTCTGG AGGATTTCCT GCAGTTCAGT
601      CTGAGGGCTG TTCGGATAAT GTAG

Quality Control:cDNA sequencing results is available upon request.

Cytokine