Text Size:AAA

Mouse IL6 / Interleukin-6 Protein

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
IL6Protein Product Information
Synonym:IL6, Il-6
Protein Construction:A DNA sequence encoding the mouse IL6 (P08505) (Phe25-Thr211) was expressed and purified.
Species:Mouse
Expressed Host:E. coli
Form & Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
IL6Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity:1. Measured by its ability to bind biotinylated rat IL6ST-mFch (Cat:80030-R06H) in a functional ELISA.
2. Measured by its ability to bind biotinylated mouse IL6ST-Fc2h (Cat:50135-M03C) in a functional ELISA.
3. Measured by its ability to bind biotinylated mouse IL6ST-His (Cat:50135-M08H) in a functional ELISA.
4. Measured by its ability to bind biotinylated human IL6RB-Fch (Cat:10974-H03H) in a functional ELISA.
5. Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells. The ED50 for this effect is typically 0.4-2 ng/ml.
Endotoxin:Please contact us for more information.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Molecule Mass:The recombinant mouse IL6 consists of 188 amino acids and predicts a molecular mass of 21.9 KDa. It migrates as an approximately 22 KDa band in SDS-PAGE under reducing conditions.
IL6Protein Usage Guide
Storage:Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
50136-MNAE
Background

Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

References
  • Heinrich PC. et al. (2003). Principles of interleukin-6-type cytokine signalling and its regulation. Biochem J. 374: 1-20.
  • Rose-John S, et al. (2007) The IL-6/sIL-6R complex as a novel target for therapeutic approaches. Expert Opin Ther Targets. 11(5): 613-24.
  • Dinh W, et al. (2009) Elevated plasma levels of TNF-alpha and interleukin-6 in patients with diastolic dysfunction and glucose metabolism disorders. Cardiovasc Diabetol. 8:58.
  • IL-6 Family & Receptor Related Products
    Product nameProduct name
    Human IL6ST / gp130 / CD130 Protein (His & Fc Tag)Human IL6ST / gp130 / CD130 ProteinMouse LIF Protein (Fc Tag)Mouse LIF Protein (His Tag)Human CNTF Protein (His Tag)Human GM-CSF / CSF2 Protein (Fc Tag)Human G-CSF / CSF3 Protein (Fc Tag)Human NNT1 / CLCF1 / CLC Protein (Fc Tag)Canine IL11RA / IL-11RA / IL11Rα ProteinRat CNTF / Ciliary Neurotrophic Factor ProteinRat CNTFR / CNTFR-alpha Protein (His Tag)Rat GM-CSF / CSF2 Protein (Fc Tag)Rat GM-CSF / CSF2 Protein (His Tag)Rat gp130 / IL6ST / CD130 Protein (His & Fc Tag)Rat gp130 / IL6ST / CD130 Protein (His Tag)Human CNTFR / CNTFR-alpha Protein (His Tag)Mouse Oncostatin M / OSM Protein (His Tag)Human Leptin ProteinHuman LIF Protein (His Tag)Rat IL6 / Interleukin-6 ProteinHuman LIF Protein (Fc Tag)Mouse Oncostatin M / OSM ProteinMouse CNTF / Ciliary Neurotrophic Factor Protein (His Tag)Human GM-CSF / CSF2 ProteinMouse IL11RA / IL11Rα Protein (His Tag)Mouse LIF ProteinHuman GM-CSF / CSF2 Protein (His Tag)Human GM-CSF / CSF2 ProteinHuman Oncostatin M / OSM Protein (His Tag)Mouse IL6 / Interleukin-6 ProteinRat LIFR Protein (His Tag)Human LIFR / CD118 Protein (His Tag)Human G-CSFR / CD114 / CSF3R Protein (Fc Tag)Human G-CSFR / CD114 Protein (His Tag)Human G-CSFR / CD114 / CSF3R ProteinHuman G-CSF / CSF3 Protein (isoform b)Human Oncostatin M / OSM ProteinCynomolgus / Rhesus IL6 / Interleukin-6 ProteinHuman IL11RA / IL11Rα Protein (His Tag)Mouse IL6ST / CD130 Protein (His & Fc Tag)Mouse IL6ST / gp130 / CD130 Protein (His & Fc Tag)Mouse IL6ST / gp130 / CD130 Protein (His Tag)Mouse IL-31 / IL31 Protein (His Tag)Rat IL-6R / CD126 Protein (ECD, His Tag)Canine IL11RA / IL-11RA / IL11Rα Protein (His Tag)Human IL6 / Interleukin-6 ProteinHuman IL-31 / IL31 Protein (His Tag)Human G-CSF / CSF3 Protein (isoform b)Human CSF2RA / GM-CSFR / CD116 Protein (His Tag)Human CSF2RA / GM-CSFR / CD116 Protein (Fc Tag)Mouse GM-CSF / CSF2 ProteinHuman Leptin Receptor / LEPR / CD295 Protein (His & Fc Tag)Human Leptin Receptor / LEPR / CD295 Protein (His Tag)Human LIF ProteinCynomolgus IL6ST / gp130 Protein (Fc Tag)Cynomolgus IL6ST / gp130 Protein (His Tag)Mouse IL6RA / CD126 Protein (His Tag)Mouse GM-CSF / CSF2 Protein (Fc Tag)Mouse GM-CSF / CSF2 Protein (His Tag)Human IL11 / Interleukin 11 / IL-11 ProteinHuman OSMR / IL31RB Protein (His Tag)Human IL6R / CD126 Protein (His Tag)Mouse LIFR / CD118 Protein (His Tag)Mouse OSMR / IL-31RB Protein (His Tag)
    Catalog:50136-MNAE-5
    List Price: $78.00  (Save $0.00)
    Price:$78.00      [How to order]
    Availabilityon Sale
    Images
    • Mouse IL6 / Interleukin-6 Protein SDS-PAGE
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"