Human IL5Ra transcript variant 1 ORF Expression Clone, FLAG-tagged (Cytokine)

IL5Ra(variant1) cDNA Clone Datasheet

IL5Ra(variant1) cDNA Clone Product Information

• Gene Bank Ref.ID: NM_000564.3
• cDNA Size: 1290
• Gene Synonym: IL5R, CD125, CDw125, HSIL5R3, MGC26560, IL5RA
• Source: Human

IL5Ra(variant1) cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens interleukin 5 receptor, alpha (IL5RA), transcript variant 1 DNA.
• Vector: pCMV/hygro-FLAG
• FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

IL5Ra(variant1) cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL5Ra(variant1) cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL5Ra(variant1) cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATCATCG TGGCGCATGT ATTACTCATC CTTTTGGGGG CCACTGAGAT ACTGCAAGCT
61       GACTTACTTC CTGATGAAAA GATTTCACTT CTCCCACCTG TCAATTTCAC CATTAAAGTT
121      ACTGGTTTGG CTCAAGTTCT TTTACAATGG AAACCAAATC CTGATCAAGA GCAAAGGAAT
181      GTTAATCTAG AATATCAAGT GAAAATAAAC GCTCCAAAAG AAGATGACTA TGAAACCAGA
241      ATCACTGAAA GCAAATGTGT AACCATCCTC CACAAAGGCT TTTCAGCAAG TGTGCGGACC
301      ATCCTGCAGA ACGACCACTC ACTACTGGCC AGCAGCTGGG CTTCTGCTGA ACTTCATGCC
361      CCACCAGGGT CTCCTGGAAC CTCAATTGTG AATTTAACTT GCACCACAAA CACTACAGAA
421      GACAATTATT CACGTTTAAG GTCATACCAA GTTTCCCTTC ACTGCACCTG GCTTGTTGGC
481      ACAGATGCCC CTGAGGACAC GCAGTATTTT CTCTACTATA GGTATGGCTC TTGGACTGAA
541      GAATGCCAAG AATACAGCAA AGACACACTG GGGAGAAATA TCGCATGCTG GTTTCCCAGG
601      ACTTTTATCC TCAGCAAAGG GCGTGACTGG CTTGCGGTGC TTGTTAACGG CTCCAGCAAG
661      CACTCTGCTA TCAGGCCCTT TGATCAGCTG TTTGCCCTTC ACGCCATTGA TCAAATAAAT
721      CCTCCACTGA ATGTCACAGC AGAGATTGAA GGAACTCGTC TCTCTATCCA ATGGGAGAAA
781      CCAGTGTCTG CTTTTCCAAT CCATTGCTTT GATTATGAAG TAAAAATACA CAATACAAGG
841      AATGGATATT TGCAGATAGA AAAATTGATG ACCAATGCAT TCATCTCAAT AATTGATGAT
901      CTTTCTAAGT ACGATGTTCA AGTGAGAGCA GCAGTGAGCT CCATGTGCAG AGAGGCAGGG
961      CTCTGGAGTG AGTGGAGCCA ACCTATTTAT GTGGGAAATG ATGAACACAA GCCCTTGAGA
1021     GAGTGGTTTG TCATTGTGAT TATGGCAACC ATCTGCTTCA TCTTGTTAAT TCTCTCGCTT
1081     ATCTGTAAAA TATGTCATTT ATGGATCAAG TTGTTTCCAC CAATTCCAGC ACCAAAAAGT
1141     AATATCAAAG ATCTCTTTGT AACCACTAAC TATGAGAAAG CTGGGTCCAG TGAGACGGAA
1201     ATTGAAGTCA TCTGTTATAT AGAGAAGCCT GGAGTTGAGA CCCTGGAGGA TTCTGTGTTT
1261     GCT1GATTACA AGGATGACGA CGATAAG2TAA

 

1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. FLAG Tag

 

 

Quality Control:cDNA sequencing results is available upon request.