Canine IL5 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
DG70019-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IL5 cDNA Clone Datasheet

IL5 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001006950.1
cDNA Size:	405
Gene Synonym:	IL5
Source:	Canine

IL5 cDNA Clone Description

cDNA Description:	ORF Clone of Canis lupus familiaris interleukin 5 (colony-stimulating factor, eosinophil) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

IL5 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL5 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL5 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGAATGC TTCTGAATTT GAGTTTGCTA GCTCTTGGGG CTGCCTATGT TTCTGCCTTT
61       GCTGTAGAAA ATCCCATGAA TAGACTGGTG GCAGAGACCT TGACACTGCT CTCCACTCAT
121      CGAACTTGGC TGATAGGCGA TGGGAACCTG ATGATTCCTA CTCCTGAAAA TAAAAATCAC
181      CAACTGTGCA TTAAAGAAGT TTTTCAGGGT ATAGACACAT TGAAGAACCA AACTGCCCAC
241      GGGGAGGCTG TGGATAAACT ATTCCAAAAC TTGTCTTTAA TAAAAGAACA CATAGAGCGC
301      CAAAAAAAAA GGTGTGCAGG AGAAAGATGG AGAGTGACAA AGTTCCTAGA CTACCTGCAA
361      GTATTTCTTG GTGTAATAAA CACCGAGTGG ACACCGGAAA GTTGA

Quality Control:cDNA sequencing results is available upon request.

Cytokine