Mouse IL5 Gene cDNA Clone / ORF Clone

IL5 cDNA Clone Datasheet

IL5 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_010558.1
• cDNA Size: 402
• Gene Synonym: IL-5
• Source: Mouse

IL5 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus interleukin 5 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

IL5 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL5 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL5 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGAAGGA TGCTTCTGCA CTTGAGTGTT CTGACTCTCA GCTGTGTCTG GGCCACTGCC
61       ATGGAGATTC CCATGAGCAC AGTGGTGAAA GAGACCTTGA CACAGCTGTC CGCTCACCGA
121      GCTCTGTTGA CAAGCAATGA GACGATGAGG CTTCCTGTCC CTACTCATAA AAATCACCAG
181      CTATGCATTG GAGAAATCTT TCAGGGGCTA GACATACTGA AGAATCAAAC TGTCCGTGGG
241      GGTACTGTGG AAATGCTATT CCAAAACCTG TCATTAATAA AGAAATACAT TGACCGCCAA
301      AAAGAGAAGT GTGGCGAGGA GAGACGGAGG ACGAGGCAGT TCCTGGATTA CCTGCAAGAG
361      TTCCTTGGTG TGATGAGTAC AGAGTGGGCA ATGGAAGGCT GA

 

Quality Control:cDNA sequencing results is available upon request.