Mouse IL5 Protein (His Tag)

Interleukin 5 Protein Datasheet

IL5 Protein Product Information

• Synonym: RP23-239O19.2, Il-5
• Protein Construction: A DNA sequence encoding the mouse IL5 (P04401) (Met 1-Gly 133) was expressed，with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Baculovirus-Insect cells

IL5 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.
  2. Immobilized recombinant mouse IL5-his at 5 μg/ml (100 μl/well) can bind biotinylated human IL5Ra-his with a linear range of 32-4000 ng/mL.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 21
• Molecular Mass: The recombinant mouse IL5 consists of 123 amino acids and has a calculated molecular mass of 14.5 kDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions, and the homodimer form could also be seen.
• Formulation: Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH 7.0, 10% gly
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL5 protein

IL5 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL5 Protein Description

Interleukins are a group of cytokines (secreted proteins/signaling molecules) that were first seen to be expressed by white blood cells (leukocytes) and has been found in a wide variety of body cells. Interleukin 5 or IL-5 is a member of the interleukin family with length of 115 amino acids. This cytokine is produced by T helper-2 cells and mast cells. It helps to stimulate B cell growth and increase immunoglobulin secretion and is considered as a key mediator in eosinophil activation. Interleukin 5 (IL-5) has long been associated with several allergic diseases, including allergic rhinitis and asthma. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. IL-5 also had something with the terminally differentiated granulocyte eosinophils. IL-5 was originally found as an eosinophil colony stimulating factor. It has been proved to be a major regulator of eosinophil accumulation in tissues, and can modulate eosinophil behavior at every stage from maturation to survival.

References

1. Milburn MV. et al., 1993, Nature. 363 (6425): 172-6.
2. Lee JS. et al., 1989, Somat Cell Mol Genet. 15 (2): 143-52.
3. Woodcock JM. et al., 1994, EMBO J. 13 (21): 5176-85.