Rat IL4R Gene cDNA Clone / ORF Clone

IL4R cDNA Clone Datasheet

IL4R cDNA Clone Product Information

• Gene Bank Ref.ID: NM_133380.2
• cDNA Size: 2406
• Gene Synonym: Il4ra, Il4r
• Source: Rat

IL4R cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus interleukin 4 receptor, alpha DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1817 T/C resulting in the amino acid val substitution by ala.

IL4R cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL4R cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL4R cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGTGGC TTTGCACCAA GTTCCTGTCC TCCGTGAGCT GTCTGATTTT GCTATGGGTG
61       ACTGGCTCTG GGGGCATCAA GGTCCTGGGT GACCCCACCT GCTTCTCTGA CTACATCCGC
121      ACTTCTACGT GTGAGTGGCA GCTGGATAGC ACTGTGGACT GCAGTTCTCA GCTCCTCCTG
181      GACTACAGGC TGCTCTTTGA GTTCTCTGAA AACCTCACAT GCACCCCCAA GAACAGTGCC
241      GACACCGTGT GTGTGTGCCA AATGGCCATA GAGGAGCCGA TCCAGGCAGA CACATACTGG
301      CTGGAACTGT GGTCTGAGCG GGGACAGCTG TGGCAAGGCT CCTTCAAGCC TAGTGACAAT
361      GTGAAGCCCC CAGCTCCAGA CAACCTCACA CTCCACACCA ATGTCTCCAA TGCGCTGCTG
421      CTGATGTGGA GCAACCCATA CCCATCGAAC AACTTCCTGC ACAAAGGCCT CATCTGCATG
481      GTCAACATCT CCAGAGAGGA CAACCCGGCG GAATTCAAAG TCTATAATGT GACCTACACG
541      GAACCCAAGC TGAGCTTCCC AGTCAACACC CTAACATCAG GGGTGCGCTA TAGGGCACGT
601      GTGAGGGTCT TGTCCCAGAG CTTCCCTGGC ATCTGGAGTG AGTGGAGTCC CAGCATCACG
661      TGGTACAACC ACTTCCAGCT GCCCCTGCTG CAGCGCCTCC CGCTGGGTGT CAGCATCTCC
721      TGCATCTGCA TCCTATTGTT TTGCCTGACC TGTTACTTCA GCATTATCAA GATTAAGAAG
781      ATATGGTGGG ACCAGATTCC CACTCCAGCA CGCAGTCCCT TAGCAGCCAT CATCATTCAG
841      GACACGAAGG TGTCCCTCTG GGAGAAGCAG ACCCGAAGCC AGGAGTCAAC CAAGAGCCGG
901      CACTGGAAGA CTTGTCTAAC CAAGCTGCTG CCCTGCTTGC TGGAGCATAG AGTGAAGAAA
961      GAGAGAGAAT CCCCGAAGGC TGCCAAAACC AAGCCTCTCC AGAGTCCTGA AAAAGCAGGC
1021     TGGTATCCTG CGGAGGTCAG CAGGACCGTC CTCTGGCCAG AGAACGTTCA TGTCAGTGTG
1081     GTGCGCTGTA TGGAGCTGTT TGAGGCCCCA GTACAGAATG TGGAGGAGGA AGAGGATGAG
1141     ATGGTCAAAG GGGACCTGAG CATGTCACCT GAGAACAGCG GAGGGGGCTT CCAGGAGAGT
1201     CAGGCAGACA TCATGGCCCG GCTCACTGAG AACCTGTTTT CAGACTTGCT GGGGGCTGAG
1261     AATGGAGGCG TTGGCCAGTC GAGCATGGCA GAGTCATCCT CCCTTCTGCC TTCAGAAAGT
1321     GGGCAAGCTT CTACGTCCTG GGCCTGCTTC CCCACGGGGC CCAGTGAGAC CACATGCCAG
1381     GTCACAGGGC AGCAGCCTCC ACACCCAGAC CCTGAGAGAG CAACTGGCAC AGCCTGCACA
1441     CAGGTCCCCC TTGTCATTTC AGACAATCCT GCCTACCGGA GTTTTAGTGA CTTCTCCAGC
1501     CCCGCCCCAA ATCCTGGAGA GCTGGCTTCA GAACAGAAGC AGGCTGGACA TCTGGAAGAA
1561     GGGGATCTTC TGAGCCCGGT TGACCCCCAT TCTTCAGGGC CACCAATGCA GCAAGCGGAG
1621     AGCTGGGAGC AGATCCTTCA CATGAGTGTC CTGCAGCACG GGACAGCTGG CTCCACCCCA
1681     GCCCCTACCA GTGGCTACCA AGAGTTTGTG CAGGCAGTGA AGCAGGGTGC GTCCCAGGAC
1741     GCTGGGGTGC CTGGTGTCGG GCCTTCTGGA GACACTGGTT ACAAGGCTTT CTCGAGCCTG
1801     CTCAGCAGCA GTGGCGCCTG CACGGACACA GCAGCAGCAG GGACTGACAG TGGATGTGGA
1861     GGGTACAAGC CCTTCCAGAA CCCTGTTCCT AACCAGTCCC CCAACTCCAT GCCCTTATTC
1921     ACCTTCGGAC TGGACATGGA GCTGCCACCC AGTCCCCTGA ACTCAGCCCC ACCCAATAGC
1981     ACCCCAGAAT GCCTTGGTCT AGAGCTGGGG CTCAAAGGAG GCACCTGGCT GAAGGCCCCT
2041     CCTCCTTCAG AGCAGGTGCC CAAGCCCTTT GGGGATGACC TGGGCCTTGG CATTGTGTAC
2101     TCGTCCCTCA CCTGCCATCT GTGTGGCCAC CTGAAGCAAC ACCACAGCCA GGAGGAAGAT
2161     GGTCAGATCC ATGTCGTTGC TAGCCCTGGC TGCGGCTGTT GCTATGATGA AAAATCACCA
2221     TCCTTGGGGA ATCTCTCGGG GACCTTGGAG AGCTGTCCTG GGGAAATGTC ACAGGAAGCC
2281     AACCTCACCT TAGCACCCAG GACACCCTCC AATTTGTCAG GGGTGGGCAA GGGCCCTGGC
2341     CACTCTCCTG TTCCCAGCCA GACTACTGAG GTACCTGTGG GCACTCTGGG CGTCACTGTG
2401     TCTTAG

 

Quality Control:cDNA sequencing results is available upon request.