Canine IL4 Gene cDNA Clone / ORF Clone (Cytokine)

IL4 cDNA Clone Datasheet

IL4 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001003159.1
• cDNA Size: 399
• Gene Synonym: IL-4；IL4
• Source: Canine

IL4 cDNA Clone Description

• cDNA Description: ORF Clone of Canis lupus familiaris interleukin 4 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

IL4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL4 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGTCTCA CCTCCCAACT GATTCCAACT CTGGTCTGCT TACTAGCACT CACCAGCACC
61       TTTGTCCACG GACATAACTT CAATATTACT ATTAAAGAGA TCATCAAAAT GTTGAACATC
121      CTCACAGCGA GAAACGACTC GTGCATGGAG CTGACTGTCA AGGACGTCTT CACTGCTCCA
181      AAGAACACAA GCGATAAGGA AATCTTCTGC AGAGCTGCTA CTGTACTGCG GCAGATCTAT
241      ACACACAACT GCTCCAACAG ATATCTCAGA GGACTCTACA GGAACCTCAG CAGCATGGCA
301      AACAAGACCT GTTCTATGAA TGAAATCAAG AAGAGTACAC TGAAAGACTT CTTGGAAAGG
361      CTAAAAGTGA TCATGCAGAA GAAATACTAC AGGCATTGA

 

Quality Control:cDNA sequencing results is available upon request.