Mouse IL4 Gene cDNA Clone / ORF Clone (Cytokine)

IL4 cDNA Clone Datasheet

IL4 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_021283.1
• cDNA Size: 423
• Gene Synonym: IL-4
• Source: Mouse

IL4 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus interleukin 4 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

IL4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL4 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGTCTCA ACCCCCAGCT AGTTGTCATC CTGCTCTTCT TTCTCGAATG TACCAGGAGC
61       CATATCCACG GATGCGACAA AAATCACTTG AGAGAGATCA TCGGCATTTT GAACGAGGTC
121      ACAGGAGAAG GGACGCCATG CACGGAGATG GATGTGCCAA ACGTCCTCAC AGCAACGAAG
181      AACACCACAG AGAGTGAGCT CGTCTGTAGG GCTTCCAAGG TGCTTCGCAT ATTTTATTTA
241      AAACATGGGA AAACTCCATG CTTGAAGAAG AACTCTAGTG TTCTCATGGA GCTGCAGAGA
301      CTCTTTCGGG CTTTTCGATG CCTGGATTCA TCGATAAGCT GCACCATGAA TGAGTCCAAG
361      TCCACATCAC TGAAAGACTT CCTGGAAAGC CTAAAGAGCA TCATGCAAAT GGATTACTCG
421      TAG

 

Quality Control:cDNA sequencing results is available upon request.