IL36G / IL1F9 (aa 18-169) Protein (His Tag) (Cytokine)

Interleukin 36, gamma Protein Datasheet

IL36G / IL1F9 Protein Product Information

• Synonym: IL1E, IL1F9, IL1H1, IL1RP2, IL1F9
• Protein Construction: A DNA sequence encoding the mature form of human IL1F9 (NP_062564) (Ser18-Asp169) was expressed with a polyhistide tag at the N-terminus.
• Source: Human
• Expression Host: E.coli

IL36G / IL1F9 Protein QC Testing

• Purity: > 98% as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human IL1F9 consists of 168 amino acids and predicts a molecular mass of 19.1 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL36G / IL1F9 protein

IL36G / IL1F9 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL36G / IL1F9 Protein Description

IL-1F9, also known as IL-36G and interleukin 36, gamma, is a member of the interleukin-1 (IL-1) family of cytokines. Interleukin-1 (IL-1) has been implicated in neuroimmune responses and has pleiotropic actions in the brain. The activity of this cytokine is mediated by interleukin 1 receptor-like 2(IL1RL2 / IL1R-rp2) IL-1F9 has been found function in activating nuclear factor- Kappa B (NF-κB) through the orphan receptor IL-1 receptor-related protein 2 (IL1R-rp2) in Jurkat cells. IL-1F9 is expressed strongly in keratinocytes and its expression is induced in skin during contact hypersensitivity and in psoriasis patients. This cytokine is also constitutively expressed primarily in the placenta and the squamous epithelium of the esophagus. The three-dimensional folding of IL-1F9 is similar to that of IL-1Ra, therefore, IL-1F9 appears to be a possible IL-1 receptor antagonist rather than an agonist.

References

1. Dinarello CA. 2002, Clin Exp Rheumatol. 20 (5): S1-13.
2. Bergl f E. et al., 2003, J Neuroimmunol. 139 (1-2): 36-43.
3. Dunn E. et al., 2001, Trends Immunol. 22 (10): 533-6.
4. Towne JE. et al., 2004, J Biol Chem. 279 (14): 13677-88.