IL36G / IL1F9 Protein (Native)

Interleukin 36, gamma Protein Datasheet

IL36G / IL1F9 Protein Product Information

• Synonym: IL36G, UNQ2456, PRO5737, IL-1F9, IL-1H1, IL-1RP2, IL1E, IL1F9, IL1H1, IL1RP2
• Protein Construction: A DNA sequence encoding the human IL1F9 isoform 1 (?Q9NZH8-1) (Met 1-Asp 169 ) was expressed.
• Source: Human
• Expression Host: E.coli

IL36G / IL1F9 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant human IL1F9 consisting of 169 amino acids and has a calculated molecular mass of 18.9KDa. It migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of PBS, pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL36G / IL1F9 protein

IL36G / IL1F9 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL36G / IL1F9 Protein Description

IL-1F9, also known as IL-36G and interleukin 36, gamma, is a member of the interleukin-1 (IL-1) family of cytokines. Interleukin-1 (IL-1) has been implicated in neuroimmune responses and has pleiotropic actions in the brain. The activity of this cytokine is mediated by interleukin 1 receptor-like 2(IL1RL2 / IL1R-rp2) IL-1F9 has been found function in activating nuclear factor- Kappa B (NF-κB) through the orphan receptor IL-1 receptor-related protein 2 (IL1R-rp2) in Jurkat cells. IL-1F9 is expressed strongly in keratinocytes and its expression is induced in skin during contact hypersensitivity and in psoriasis patients. This cytokine is also constitutively expressed primarily in the placenta and the squamous epithelium of the esophagus. The three-dimensional folding of IL-1F9 is similar to that of IL-1Ra, therefore, IL-1F9 appears to be a possible IL-1 receptor antagonist rather than an agonist.

References

1. Dinarello CA. 2002, Clin Exp Rheumatol. 20 (5): S1-13.
2. Bergl f E. et al., 2003, J Neuroimmunol. 139 (1-2): 36-43.
3. Dunn E. et al., 2001, Trends Immunol. 22 (10): 533-6.
4. Towne JE. et al., 2004, J Biol Chem. 279 (14): 13677-88.