IL36B / IL1F8 Protein (Native) (Cytokine)

Interleukin 36, beta Protein Datasheet

IL36B / IL1F8 Protein Product Information

• Synonym: IL36B , FIL1, FIL1-(ETA), FIL1H, FILI-(ETA), IL-1F8, IL-1H2, IL1-ETA, IL1F8, IL1H2, MGC126880, MGC126882
• Protein Construction: A DNA sequence encoding the mature form of human IL36B isofrom 2 (Q9NZH7-2) (Met 1-Glu 157) was expressed.
• Source: Human
• Expression Host: E.Coli

IL36B / IL1F8 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human IL36B consists of 157 amino acids and has a predicted molecular mass of 17.7 KDa. It migrates as an approximately 18KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL36B / IL1F8 protein

IL36B / IL1F8 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL36B / IL1F8 Protein Description

Interleukin-1 family member 8(IL1F8) also known as IL36B, is a member of the interleukin 1(IL-1) cytokine family. IL1F8 may contains a 12-stranded beta-trefoil structure. Until recently, the IL-1 family of cytokines includ four members, with three having pro-inflammatory effects such as IL1F8 and the fourth member being an IL-1 receptor antagonist (IL-1Ra). IL-1 family members exert their effects through binding to receptors that belong to the IL-1 receptor (IL-1R) family. IL1F8 exerts proinflammatory effects in primary human joint cells. Joint and serum IL-1F8 protein levels did not correlate with inflammation, but they were high in some human serum samples tested, including samples from patients with rheumatoid arthritis. IL1F8 also activated NK-κB.

References

1. Magne D, et al. (2006) Arthritis Research & Therapy. 8:R80.
2. Smith DE, et al. (2000) The Journal of Biological Chemistry. 275: 1169-75.